TY - JOUR
T1 - In vivo quantitative three-dimensional localization of tumor labeled with exogenous specific fluorescence markers
AU - Gannot, Israel
AU - Garashi, Avital
AU - Gannot, Gallya
AU - Chernomordik, Victor
AU - Gandjbakhche, Amir
PY - 2003/6/1
Y1 - 2003/6/1
N2 - We introduce a diffused optical detection system based on the administration of a fluorophore–antibody conjugate to diseased tissue. The conjugate interacts with the antigens expressed by the diseased tissue, resulting in fluorescent labeling of the antigen. By combining an optical detection system with a reconstruction algorithm developed on the basis of the random-walk model, we were able to determine the position of the fluorophore (and, thus, of the diseased cells) in the tissue. We present three–dimensional reconstructions of the location of a fluorophore (FITC–fluorescein isothiocyanate) in the tongues of mice. Measurements were performed with the fluorophore embedded at various simulated depths. The simulations were performed with agarose-based gel slabs applied to the tongue as tissuelike phantoms. Reconstructed fluorophore locations agree well with the actual values.
AB - We introduce a diffused optical detection system based on the administration of a fluorophore–antibody conjugate to diseased tissue. The conjugate interacts with the antigens expressed by the diseased tissue, resulting in fluorescent labeling of the antigen. By combining an optical detection system with a reconstruction algorithm developed on the basis of the random-walk model, we were able to determine the position of the fluorophore (and, thus, of the diseased cells) in the tissue. We present three–dimensional reconstructions of the location of a fluorophore (FITC–fluorescein isothiocyanate) in the tongues of mice. Measurements were performed with the fluorophore embedded at various simulated depths. The simulations were performed with agarose-based gel slabs applied to the tongue as tissuelike phantoms. Reconstructed fluorophore locations agree well with the actual values.
UR - http://www.scopus.com/inward/record.url?scp=0037871960&partnerID=8YFLogxK
U2 - 10.1364/AO.42.003073
DO - 10.1364/AO.42.003073
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AN - SCOPUS:0037871960
SN - 1559-128X
VL - 42
SP - 3073
EP - 3080
JO - Applied Optics
JF - Applied Optics
IS - 16
ER -