In vivo expression of inducible nitric oxide synthase in cerebellar neurons

Dahlia Minc-Golomb, Gal Yadid, Ilan Tsarfaty, James H. Resau, Joan P. Schwartz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


In the CNS, nitric oxide (NO) functions as both neuromodulator and neurotoxic agent. In vivo neuronal expression of NO synthase (NOS) has been attributed to constitutive NOS-both the neuronal and the endothelial types. The other class of NOS-the inducible NOS (iNOS)-is known to mediate toxic effects of NO in various tissues. In this study, we show for the first time that direct intracerebellar injection of endotoxin and cytokine (lipopolysaccharide and interferon-γ) induced in vivo neuronal expression of the iNOS gene, as demonstrated by fluorescent in situ hybridization and immunohistochemical staining analyzed by confocal laser-scanning microscopy. This raises the possibility that neuronal iNOS might contribute significantly to the vulnerability of the brain to various insults.

Original languageEnglish
Pages (from-to)1504-1509
Number of pages6
JournalJournal of Neurochemistry
Issue number4
StatePublished - Apr 1996


  • Cerebellum
  • Cytokine
  • Endotoxin
  • Immunohistochemistry
  • In situ hybridization
  • Neuronal induction
  • Nitric oxide
  • Nitric oxide synthase


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