In-vivo dehydration sensing in transgenic tobacco plants using an integrated electrochemical chip

Dayananda Desagani, Aakash Jog, Adi Avni, Yosi Shacham-Diamand

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

In this paper, we demonstrate in-vivo plant based dehydration sensing using a bio-electrochemical sensor. In-vivo sensing reports on the plant's status as provided by its gene expression, responding to stress. Plant based sensing provides precise, real-time information acquired from the plants themselves; it complements the information provided by ex-vivo sensors sampling the plant's surroundings, e.g. in the soil, or sensors measuring the plant's electrical conductivity. In this paper, we present a method in which the plant's dehydration levels are monitored in real time using in-vivo techniques using the plant as the sensor. In this method, the expression of the β-Dglucuronidase enzyme, expressed under drought conditions, is monitored using its reaction with a substrate, which produces an electrochemically active product. The product is oxidized on the working electrode of a three-electrode electrochemical chip mounted on the leaves of Nicotiana tabacum plants. Electrochemical sensing showed earlier detection compared to other methods, e.g. visual inspection and conductivity measurements.

Original languageEnglish
Title of host publication2020 IEEE International Symposium on Circuits and Systems, ISCAS 2020 - Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)9781728133201
StatePublished - 2020
Event52nd IEEE International Symposium on Circuits and Systems, ISCAS 2020 - Virtual, Online
Duration: 10 Oct 202021 Oct 2020

Publication series

NameProceedings - IEEE International Symposium on Circuits and Systems
Volume2020-October
ISSN (Print)0271-4310

Conference

Conference52nd IEEE International Symposium on Circuits and Systems, ISCAS 2020
CityVirtual, Online
Period10/10/2021/10/20

Keywords

  • Biosensors
  • Drought plants
  • Electrochemical sensing
  • In-vivo plant sensing

Fingerprint

Dive into the research topics of 'In-vivo dehydration sensing in transgenic tobacco plants using an integrated electrochemical chip'. Together they form a unique fingerprint.

Cite this