In vitro synthesis of hemocyanin

1. 1. In vitro synthesized hemocyanin was identified among the products of cell-free translation of poly (A) mRNA isolated from whole Leiurus quinquestriatus scorpions. 2. 2. The heterogeneity of the polypeptide chains of native hemocyanin is reflected in the in vitro synthesized protein. It is concluded that different chains are coded by different messages and not obtained from a single type of polypeptide chain through post-translational events. 3. 3. Hemocyanin could not be detected among the products of translation of mRNA from scorpion hemolymph, ruling out the possibility that hemocyanin is synthesized in circulating cells.