In vitro synthesis of 'amyloid' fibrils from insulin, calcitonin and parathormone

I. Kedar Keizman, M. Ravid, E. Sohar

Research output: Contribution to journalArticlepeer-review

Abstract

Insulin, calcitonin and parathyroid hormone subjected to 1 of 2 procedures, acidification and heating or incubation with mouse kidney lysosomal extracts, assumed a nonbranching fibrillar structure, 7 to 10 nm in diameter. The preparations showed green birefringence after Congo red staining. The in vitro synthesis from different hormonal polypeptides of fibrils, fulfilling the criteria for the identification of amyloid, indicates that these criteria are related to conformational rather than to compositional properties, and suggests that these hormones may provide the subunit of the amyloid formed in the corresponding endocrine organs.

Original languageEnglish
Pages (from-to)1137-1140
Number of pages4
JournalIsrael Journal of Medical Sciences
Volume12
Issue number10
StatePublished - 1976
Externally publishedYes

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