TY - JOUR
T1 - In vitro synthesis of 'amyloid' fibrils from insulin, calcitonin and parathormone
AU - Kedar Keizman, I.
AU - Ravid, M.
AU - Sohar, E.
PY - 1976
Y1 - 1976
N2 - Insulin, calcitonin and parathyroid hormone subjected to 1 of 2 procedures, acidification and heating or incubation with mouse kidney lysosomal extracts, assumed a nonbranching fibrillar structure, 7 to 10 nm in diameter. The preparations showed green birefringence after Congo red staining. The in vitro synthesis from different hormonal polypeptides of fibrils, fulfilling the criteria for the identification of amyloid, indicates that these criteria are related to conformational rather than to compositional properties, and suggests that these hormones may provide the subunit of the amyloid formed in the corresponding endocrine organs.
AB - Insulin, calcitonin and parathyroid hormone subjected to 1 of 2 procedures, acidification and heating or incubation with mouse kidney lysosomal extracts, assumed a nonbranching fibrillar structure, 7 to 10 nm in diameter. The preparations showed green birefringence after Congo red staining. The in vitro synthesis from different hormonal polypeptides of fibrils, fulfilling the criteria for the identification of amyloid, indicates that these criteria are related to conformational rather than to compositional properties, and suggests that these hormones may provide the subunit of the amyloid formed in the corresponding endocrine organs.
UR - http://www.scopus.com/inward/record.url?scp=0017040042&partnerID=8YFLogxK
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AN - SCOPUS:0017040042
SN - 0021-2180
VL - 12
SP - 1137
EP - 1140
JO - Israel Journal of Medical Sciences
JF - Israel Journal of Medical Sciences
IS - 10
ER -