In vitro simulation of placental transport: Part I. Biological model of the placental barrier

R. Levkovitz; U. Zaretsky; Z. Gordon; A. J. Jaffa; D. Elad

doi:10.1016/j.placenta.2013.03.014

In vitro simulation of placental transport: Part I. Biological model of the placental barrier

R. Levkovitz, U. Zaretsky, Z. Gordon, A. J. Jaffa, D. Elad^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

Introduction: The placental barrier (PB) is the thin biological membrane made of endothelial cells (EC), trophoblast cells (TC) and basal membrane that separates between maternal and fetal blood circulations within the placenta and facilitates feto-maternal transport characteristics, which are not completely understood. Methods: An in vitro biological model of the PB model was co-cultured of human TC (HTR8) and human umbilical vein EC (HUVEC) on both sides of a denuded amniotic membrane (AM) using custom designed wells. Results: Confocal and transmission electron microscopy (TEM) imaging confirmed the morphology expressions of human EC and TC. Further support on the integrity of the new PB model was obtained from the existence of tight junctions and permeability experiments with fluorescence markers of small and large molecules. The monolayer of EC demonstrated the limiting layer for the transport resistance across this complex barrier. Discussion and conclusion: This new in vitro viable model mimics the architecture of the human PB and can be used in in vitro simulations of transplacental transport studies.

Original language	English
Pages (from-to)	699-707
Number of pages	9
Journal	Placenta
Volume	34
Issue number	8
DOIs	https://doi.org/10.1016/j.placenta.2013.03.014
State	Published - Aug 2013

Funding

Funders	Funder number
Gesellschaft der Freunde der Universität Tel Aviv in Österreich
Nicholas and Elizabeth Slezak Super Center for Cardiac Research and Biomedical Engineering

Keywords

Amniotic membrane
Co-culture
Feto-maternal transport
Permeability coefficient
Tissue engineered placental barrier

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10.1016/j.placenta.2013.03.014

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title = "In vitro simulation of placental transport: Part I. Biological model of the placental barrier",

abstract = "Introduction: The placental barrier (PB) is the thin biological membrane made of endothelial cells (EC), trophoblast cells (TC) and basal membrane that separates between maternal and fetal blood circulations within the placenta and facilitates feto-maternal transport characteristics, which are not completely understood. Methods: An in vitro biological model of the PB model was co-cultured of human TC (HTR8) and human umbilical vein EC (HUVEC) on both sides of a denuded amniotic membrane (AM) using custom designed wells. Results: Confocal and transmission electron microscopy (TEM) imaging confirmed the morphology expressions of human EC and TC. Further support on the integrity of the new PB model was obtained from the existence of tight junctions and permeability experiments with fluorescence markers of small and large molecules. The monolayer of EC demonstrated the limiting layer for the transport resistance across this complex barrier. Discussion and conclusion: This new in vitro viable model mimics the architecture of the human PB and can be used in in vitro simulations of transplacental transport studies.",

keywords = "Amniotic membrane, Co-culture, Feto-maternal transport, Permeability coefficient, Tissue engineered placental barrier",

author = "R. Levkovitz and U. Zaretsky and Z. Gordon and Jaffa, {A. J.} and D. Elad",

note = "Funding Information: This research was partially supported by the Australian Friends of Tel Aviv University (Victoria) , and the Nicholas and Elizabeth Slezak Super Center for Cardiac Research and Biomedical Engineering in Tel Aviv University. We wish to thank Dr. Sorina Grisaru-Granovsky from Shaare Zedek Medical Center in Jerusalem for kindly providing the trophoblast cell line (HTR8). We wish to thank Profs. Moshe Hod, Yariv Yogev and Nir Melamed from Rabin Medical Center and Prof. Shmuel Einav from Tel Aviv University for valuable discussions and continuous support.",

year = "2013",

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doi = "10.1016/j.placenta.2013.03.014",

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AU - Elad, D.

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In vitro simulation of placental transport: Part I. Biological model of the placental barrier

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