In vitro shoot regeneration from leaf explants of Vitis vinifera (L.)

An in vitro regeneration protocol has been developed from 5-8 mm long leaf discs excised from in vitro grown regenerants as well as one-year-old plants of three different cultivars of Vitis vinifera L viz: Thompson seedless, Karnet and Local to find out the best in vitro responsive cultivar, culture media combination (especially appropriate auxin/cytokinin ratio) with their interactive effects on frequency of callus induction, formation of morphogenc calli and plantlet regeneration. MS medium fortified with 2.0-3.0 mg/1 2, 4-D and 0.5 mg/1 Kinetin or BA proved more responsive for callus induction, while morphogenic calli as well as plantlets in higher frequencies were investigated with culture medium amended with 2.0 mg/1 BA in conjunction with 0.5 mg/INAA. In term of genotypic response, genotype Thompson Seedless responded better than Karnet and Local. Higher in vitro rooting response i.e. root proliferation ability, number (s) of roots and root length was exhibited by MS rooting medium fortified with 2.0 mg/IIBA and 15 g.1^-1 sucrose. The in vitro raised plantlets were acclimatized and established successfully in the field.