In vitro selective effect of melphalan on human T-cell populations

Shlomo Ben-Efraim*, Luise Komlos, Jaffa Notmann, Jacob Hart, Isaac Halbrecht

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In vitro treatment with 2 μg/2×106 cells melphalan (l-PAM: l-phenylalanine mustard) significantly decreased the total number of T lymphocytes from peripheral blood (PBL) of healthy human donors and of the OKT4 population (precursor suppressor/helper/inducer) T cells as defined by monoclonal antibodies OKT3 and OKT4, respectively. No changes in the OKT8+lymphocyte population (cytotoxic/mature suppressor cells) were observed following the same treatment. Preincubation of PBL with l-PAM at concentrations that do not affect the rate of DNA synthesis in PHA-stimulated lymphocytes inhibited the generation of T suppressor lymphocytes by ConA, as shown by their effect on PHA stimulation. Treatment of allogeneic PBL with l-PAM had no effect on mature suppressor T cells already induced by Con A, as shown by incubation of PBL with l-PAM after incubation with ConA.

Original languageEnglish
Pages (from-to)53-56
Number of pages4
JournalCancer Immunology, Immunotherapy
Volume19
Issue number1
DOIs
StatePublished - Feb 1985
Externally publishedYes

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