TY - JOUR
T1 - In vitro preliminary evaluation of bacterial attachment on grooved and smooth healing abutments
AU - Moses, Ofer
AU - Nemcovsky, Carlos E.
AU - Lewinstein, Israel
AU - Zoabi, Hasan
AU - Weinreb, Miron
AU - Levartovsky, Shifra
AU - Matalon, Shlomo
N1 - Publisher Copyright:
© 2020 by the authors.
PY - 2020/7/1
Y1 - 2020/7/1
N2 - This in vitro preliminary study investigated the attachment of Fusobacterium nucleatum and Porphyromonas gingivalis on titanium alloy healing abutments, which differed in their surface macro-morphology: one was groove-marked while the other was completely smooth. Altogether, twenty implant-healing abutments, ten of each macro-morphology, were evaluated with a single type of bacterial strain. Accordingly, four groups of five abutments each were created. The sterilized healing abutments with the cultured bacteria were placed under anaerobic conditions for 48 h at 37 °C. Afterwards, the abutments were examined with a scanning electron microscope, at a 2500x magnification. Attached bacteria were quantified in the four vertical quarters within the grooved abutments and in the two most coronal millimeters of the smooth abutments. The results were analyzed by applying two-way ANOVA, with square root transformation for a normal distribution. The bacterial attachment of both strains was statistically significantly larger in the grooved abutment areas than on the smooth surfaces (p ≤ 0.0001), twenty times so for Porphyromonas gingivalis and a hundred times so for Fusobacterium nucleatum.
AB - This in vitro preliminary study investigated the attachment of Fusobacterium nucleatum and Porphyromonas gingivalis on titanium alloy healing abutments, which differed in their surface macro-morphology: one was groove-marked while the other was completely smooth. Altogether, twenty implant-healing abutments, ten of each macro-morphology, were evaluated with a single type of bacterial strain. Accordingly, four groups of five abutments each were created. The sterilized healing abutments with the cultured bacteria were placed under anaerobic conditions for 48 h at 37 °C. Afterwards, the abutments were examined with a scanning electron microscope, at a 2500x magnification. Attached bacteria were quantified in the four vertical quarters within the grooved abutments and in the two most coronal millimeters of the smooth abutments. The results were analyzed by applying two-way ANOVA, with square root transformation for a normal distribution. The bacterial attachment of both strains was statistically significantly larger in the grooved abutment areas than on the smooth surfaces (p ≤ 0.0001), twenty times so for Porphyromonas gingivalis and a hundred times so for Fusobacterium nucleatum.
KW - Bacterial growth
KW - Grooved healing abutment
KW - SEM
KW - Smooth healing abutment
UR - http://www.scopus.com/inward/record.url?scp=85087879335&partnerID=8YFLogxK
U2 - 10.3390/app10134426
DO - 10.3390/app10134426
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:85087879335
SN - 2076-3417
VL - 10
JO - Applied Sciences (Switzerland)
JF - Applied Sciences (Switzerland)
IS - 13
M1 - 4426
ER -