In Vitro Hemopoiesis of Marrow Cells from Myelodysplastic Patients: Effects of Mitogen-stimulated Spleen Cell-conditioned Medium and Vitamin A and D Derivatives

I. Fabian, I. Riklis, I. Tatarsky, A. Nagler

Research output: Contribution to journalArticlepeer-review

Abstract

We determined the ability of Pokeweed mitogen-stimulated human spleen cells to support the growth and Proliferation of hemopoietic Progenitors from normal and myelodysplastic syndrome (MDS) Patients, in the Presence and absence of the maturation-inducing agents 13-cis-retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (vit D). The addition of various concentrations of vit D or RA to culture Plates containing MDS Patients9 marrow cells stimulated myeloid colony growth. A higher number of myeloid colony-forming units-cell was noted in cultures containing vit D or RA in addition to Pokeweed mitogen spleen-conditioned medium compared to these substances and Placenta conditioned medium. When colony-forming units-cell from MDS Patients was incubated with 2 x 10-8 M vit D the Percentage of monocyte-macrophage colonies was increased and the number of granulocyte colonies was decreased. Neither vit D nor RA affected the number of cells Per colony. Our findings suggest that Pokeweed mitogen spleen-conditioned medium Provides A more Potent source than Placenta-conditioned medium for humoral factors that stimulate the growth of hemopoietic Progenitors from MDS Patients and that the use of Pokeweed mitogen spleen-conditioned medium may improve studies of hemopoiesis in MDS Patients. copyright.

Original languageEnglish
Pages (from-to)2596-2599
Number of pages4
JournalCancer Research
Volume46
Issue number5
StatePublished - 1 May 1986

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