In vitro enzyme evolution: The screening challenge of isolating the one in a million

Noa Cohen, Simona Abramov, Yael Dror, Amihay Freeman

Research output: Contribution to journalReview articlepeer-review

Abstract

The generation of large mutant libraries for in vitro enzyme evolution presents the challenge of effectively screening libraries of 104-107 mutants on the basis of simultaneously assaying their biocatalytic activity. In this review, we highlight the main steps involved in this process, describe the alternative approaches to address this challenge, survey the state-of-the-art technology and assess achievements already made. It is anticipated that, as a result of the expected accomplishment of further improvements in high-throughput screening that will allow routine screening of whole libraries, the number of useful new and improved enzymes derived through in vitro enzyme evolution will expand rapidly in the near future.

Original languageEnglish
Pages (from-to)507-510
Number of pages4
JournalTrends in Biotechnology
Volume19
Issue number12
DOIs
StatePublished - 1 Dec 2001

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