In vitro effect of ciprofloxacin on HT-29 human colon carcinoma cell line: Assessment of cell proliferation by thymidine uptake and silver nucleolar organizer regions (AgNOR) histomorphometry

Micha Rabau; Abraham Nyska; Dan Dayan

doi:10.1007/BF02733673

In vitro effect of ciprofloxacin on HT-29 human colon carcinoma cell line: Assessment of cell proliferation by thymidine uptake and silver nucleolar organizer regions (AgNOR) histomorphometry

Micha Rabau, Abraham Nyska, Dan Dayan^*

^*Corresponding author for this work

Clinical Departments

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In an in vitro study, the effect of ciprofloxacin was examined on proliferation of HT-29 human colon carcinoma cell line. Cell proliferation was measured by³H-thymidine uptake and silver nucleolar organizer region (AgNOR) histomorphometry. A significant increase in [³H]thymidine incorporation (p<0.05) parallel with a significant increase in the mean AgNOR clusters/cells (p<0.01) was noted after 5 days of cell exposure to ciprofloxacin. It is suggested that the assessment of AgNOR variables is useful in examination of quinolone effect on HT-29 human colon carcinoma cell proliferation.

Original language	English
Pages (from-to)	124-126
Number of pages	3
Journal	Archives of Toxicology
Volume	70
Issue number	2
DOIs	https://doi.org/10.1007/BF02733673
State	Published - Dec 1995

Keywords

Ciprofloxacin
Histomorphometry
Human colen carcinoma cell line
Silver nucleolar organizer regions
Thymidine uptake

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/BF02733673

Cite this

@article{3e52410c227b4cbe8207fc1c40f554a6,

title = "In vitro effect of ciprofloxacin on HT-29 human colon carcinoma cell line: Assessment of cell proliferation by thymidine uptake and silver nucleolar organizer regions (AgNOR) histomorphometry",

abstract = "In an in vitro study, the effect of ciprofloxacin was examined on proliferation of HT-29 human colon carcinoma cell line. Cell proliferation was measured by3H-thymidine uptake and silver nucleolar organizer region (AgNOR) histomorphometry. A significant increase in [3H]thymidine incorporation (p<0.05) parallel with a significant increase in the mean AgNOR clusters/cells (p<0.01) was noted after 5 days of cell exposure to ciprofloxacin. It is suggested that the assessment of AgNOR variables is useful in examination of quinolone effect on HT-29 human colon carcinoma cell proliferation.",

keywords = "Ciprofloxacin, Histomorphometry, Human colen carcinoma cell line, Silver nucleolar organizer regions, Thymidine uptake",

author = "Micha Rabau and Abraham Nyska and Dan Dayan",

year = "1995",

month = dec,

doi = "10.1007/BF02733673",

language = "אנגלית",

volume = "70",

pages = "124--126",

journal = "Archives of Toxicology",

issn = "0340-5761",

publisher = "Springer Verlag",

number = "2",

}

TY - JOUR

T1 - In vitro effect of ciprofloxacin on HT-29 human colon carcinoma cell line

T2 - Assessment of cell proliferation by thymidine uptake and silver nucleolar organizer regions (AgNOR) histomorphometry

AU - Rabau, Micha

AU - Nyska, Abraham

AU - Dayan, Dan

PY - 1995/12

Y1 - 1995/12

N2 - In an in vitro study, the effect of ciprofloxacin was examined on proliferation of HT-29 human colon carcinoma cell line. Cell proliferation was measured by3H-thymidine uptake and silver nucleolar organizer region (AgNOR) histomorphometry. A significant increase in [3H]thymidine incorporation (p<0.05) parallel with a significant increase in the mean AgNOR clusters/cells (p<0.01) was noted after 5 days of cell exposure to ciprofloxacin. It is suggested that the assessment of AgNOR variables is useful in examination of quinolone effect on HT-29 human colon carcinoma cell proliferation.

AB - In an in vitro study, the effect of ciprofloxacin was examined on proliferation of HT-29 human colon carcinoma cell line. Cell proliferation was measured by3H-thymidine uptake and silver nucleolar organizer region (AgNOR) histomorphometry. A significant increase in [3H]thymidine incorporation (p<0.05) parallel with a significant increase in the mean AgNOR clusters/cells (p<0.01) was noted after 5 days of cell exposure to ciprofloxacin. It is suggested that the assessment of AgNOR variables is useful in examination of quinolone effect on HT-29 human colon carcinoma cell proliferation.

KW - Ciprofloxacin

KW - Histomorphometry

KW - Human colen carcinoma cell line

KW - Silver nucleolar organizer regions

KW - Thymidine uptake

UR - http://www.scopus.com/inward/record.url?scp=0029598425&partnerID=8YFLogxK

U2 - 10.1007/BF02733673

DO - 10.1007/BF02733673

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 8773185

AN - SCOPUS:0029598425

SN - 0340-5761

VL - 70

SP - 124

EP - 126

JO - Archives of Toxicology

JF - Archives of Toxicology

IS - 2

ER -

In vitro effect of ciprofloxacin on HT-29 human colon carcinoma cell line: Assessment of cell proliferation by thymidine uptake and silver nucleolar organizer regions (AgNOR) histomorphometry

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this