TY - JOUR
T1 - In vitro activity of BaH1, the main hemorrhagic toxin of Bothrops asper snake venom on bovine endothelial cells
AU - Borkow, Gadi
AU - Gutiérrez, JoséMaria
AU - Ovadia, Michael
N1 - Funding Information:
study was supported by AID Grant No. DHR-5544-6-00-1064-00.
PY - 1995/10
Y1 - 1995/10
N2 - Incubation of BaH1, the main hemorrhagic toxin purified from the venom of Bothrops asper, with endothelial cells caused the appearance of spaces among the cells. This effect became more noticeable with increasing hemorrhagin concentration and longer incubation time. Later, the cells became rounded and detached from the substrate into the medium. Augmentation of Trypan blue did not stain the detached cells, indicating their viability. Moreover, after washing the floating cells from the toxin they could be recultivated: they again spread on the substrate and proliferated, demonstrating that BaH1 is not directly cytotoxic to the endothelial cells.
AB - Incubation of BaH1, the main hemorrhagic toxin purified from the venom of Bothrops asper, with endothelial cells caused the appearance of spaces among the cells. This effect became more noticeable with increasing hemorrhagin concentration and longer incubation time. Later, the cells became rounded and detached from the substrate into the medium. Augmentation of Trypan blue did not stain the detached cells, indicating their viability. Moreover, after washing the floating cells from the toxin they could be recultivated: they again spread on the substrate and proliferated, demonstrating that BaH1 is not directly cytotoxic to the endothelial cells.
UR - http://www.scopus.com/inward/record.url?scp=0028818432&partnerID=8YFLogxK
U2 - 10.1016/0041-0101(95)00078-Z
DO - 10.1016/0041-0101(95)00078-Z
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AN - SCOPUS:0028818432
SN - 0041-0101
VL - 33
SP - 1387
EP - 1391
JO - Toxicon
JF - Toxicon
IS - 10
ER -