TY - JOUR
T1 - In situ hybridization
T2 - A simple and sensitive method for detection of trisomy 12 in chronic lymphocytic leukemia
AU - Lalkin, Arie
AU - Lishner, Michael
AU - Gaber, Elena
AU - Manor, Yosef
AU - Fejgin, Moshe
AU - Ravid, Mordchai
AU - Amiel, Aliza
PY - 1993/10/1
Y1 - 1993/10/1
N2 - Chromosome aberrations are detected in only 50% of patients with chronic lymphocytic leukemia (CLL), owing usually to the low mitotic rate exhibited by the neoplastic lymphocytes. Fluorescence in situ hybridization (FISH) is a simple method for identifying numerical abnormalities of the target chromosome in interphase nuclei. Therefore, we used the FISH procedure with chromosome 12-specific α-satellite probe to evaluate 19 patients with CLL. Trisomy 12 was detected in interphase cells of 12 patients (63%). Cytogenetic analysis, performed in nine patients, yielded trisomy 12 in four (44%). FISH detected three patients with trisomy 12 in whom conventional cytogenetic method yielded a normal karyotype. FISH is a simple, reliable, and sensitive method for detection of trisomy 12 in patients with CLL.
AB - Chromosome aberrations are detected in only 50% of patients with chronic lymphocytic leukemia (CLL), owing usually to the low mitotic rate exhibited by the neoplastic lymphocytes. Fluorescence in situ hybridization (FISH) is a simple method for identifying numerical abnormalities of the target chromosome in interphase nuclei. Therefore, we used the FISH procedure with chromosome 12-specific α-satellite probe to evaluate 19 patients with CLL. Trisomy 12 was detected in interphase cells of 12 patients (63%). Cytogenetic analysis, performed in nine patients, yielded trisomy 12 in four (44%). FISH detected three patients with trisomy 12 in whom conventional cytogenetic method yielded a normal karyotype. FISH is a simple, reliable, and sensitive method for detection of trisomy 12 in patients with CLL.
UR - http://www.scopus.com/inward/record.url?scp=0027514810&partnerID=8YFLogxK
U2 - 10.1016/0165-4608(93)90126-7
DO - 10.1016/0165-4608(93)90126-7
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AN - SCOPUS:0027514810
SN - 0165-4608
VL - 70
SP - 21
EP - 24
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -
