@article{708df7a873a94933b1e30a30fc878fb2,
title = "In situ forming hydrogels composed of oxidized high molecular weight hyaluronic acid and gelatin for nucleus pulposus regeneration",
abstract = "Encapsulation of nucleus pulposus (NP) cells within in situ forming hydrogels is a novel biological treatment for early stage intervertebral disc degeneration. The procedure aims to prolong the life of the degenerating discs and to regenerate damaged tissue. In this study we developed an injectable oxidized hyaluronic acid-gelatin-Adipic acid dihydrazide (oxi-HAG-ADH) hydrogel. High molecular weight (1900 kDa) hyaluronic acid was crosslinked with various concentrations of gelatin to synthesize the hydrogels and their viscoelastic properties were analyzed. Interactions between the hydrogels, NP cells, and the extracellular matrix (ECM) were also evaluated, as were the effects of the hydrogels on NP cell gene expression. The hydrogels possess several clinical advantages, including sterilizability, low viscosity for injection, and ease of use. The viscoelastic properties of the hydrogels were similar to native tissue, as reflected in the complex shear modulus (∼11-14 kPa for hydrogels, 11.3 kPa for native NP). Cultured NP cells not only attached to the hydrogels but also survived, proliferated, and maintained their round morphology. Importantly, we found that hydrogels increased NP cell expression of several crucial ECM-related genes, such as COL2A1, AGN, SOX-9, and HIF-1A.",
keywords = "Gelatin, Gene expression, Hyaluronic acid, Nucleus pulposus, Viscoelastic properties",
author = "Chen, {Yu Chun} and Su, {Wen Yu} and Yang, {Shu Hua} and Amit Gefen and Lin, {Feng Huei}",
note = "Funding Information: This study was financially supported by National Health Research Institutes ( 00A1-MEPP01-014 ). The authors would like to thank the DNA Sequence Core Laboratory of NHRI for use of the Applied Biosystems 7900 Real-time PCR System, and the Industrial Technology Research Institute for the use of the HAKKE rheometer. ",
year = "2013",
month = feb,
doi = "10.1016/j.actbio.2012.09.039",
language = "אנגלית",
volume = "9",
pages = "5181--5193",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Acta Materialia Inc",
number = "2",
}