TY - JOUR
T1 - In situ electrochemical generation of nitric oxide for neuronal modulation
AU - Park, Jimin
AU - Jin, Kyoungsuk
AU - Sahasrabudhe, Atharva
AU - Chiang, Po Han
AU - Maalouf, Joseph H.
AU - Koehler, Florian
AU - Rosenfeld, Dekel
AU - Rao, Siyuan
AU - Tanaka, Tomo
AU - Khudiyev, Tural
AU - Schiffer, Zachary J.
AU - Fink, Yoel
AU - Yizhar, Ofer
AU - Manthiram, Karthish
AU - Anikeeva, Polina
N1 - Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Understanding the function of nitric oxide, a lipophilic messenger in physiological processes across nervous, cardiovascular and immune systems, is currently impeded by the dearth of tools to deliver this gaseous molecule in situ to specific cells. To address this need, we have developed iron sulfide nanoclusters that catalyse nitric oxide generation from benign sodium nitrite in the presence of modest electric fields. Locally generated nitric oxide activates the nitric oxide-sensitive cation channel, transient receptor potential vanilloid family member 1 (TRPV1), and the latency of TRPV1-mediated Ca2+ responses can be controlled by varying the applied voltage. Integrating these electrocatalytic nanoclusters with multimaterial fibres allows nitric oxide-mediated neuronal interrogation in vivo. The in situ generation of nitric oxide in the ventral tegmental area with the electrocatalytic fibres evoked neuronal excitation in the targeted brain region and its excitatory projections. This nitric oxide generation platform may advance mechanistic studies of the role of nitric oxide in the nervous system and other organs.
AB - Understanding the function of nitric oxide, a lipophilic messenger in physiological processes across nervous, cardiovascular and immune systems, is currently impeded by the dearth of tools to deliver this gaseous molecule in situ to specific cells. To address this need, we have developed iron sulfide nanoclusters that catalyse nitric oxide generation from benign sodium nitrite in the presence of modest electric fields. Locally generated nitric oxide activates the nitric oxide-sensitive cation channel, transient receptor potential vanilloid family member 1 (TRPV1), and the latency of TRPV1-mediated Ca2+ responses can be controlled by varying the applied voltage. Integrating these electrocatalytic nanoclusters with multimaterial fibres allows nitric oxide-mediated neuronal interrogation in vivo. The in situ generation of nitric oxide in the ventral tegmental area with the electrocatalytic fibres evoked neuronal excitation in the targeted brain region and its excitatory projections. This nitric oxide generation platform may advance mechanistic studies of the role of nitric oxide in the nervous system and other organs.
UR - http://www.scopus.com/inward/record.url?scp=85087014866&partnerID=8YFLogxK
U2 - 10.1038/s41565-020-0701-x
DO - 10.1038/s41565-020-0701-x
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C2 - 32601446
AN - SCOPUS:85087014866
SN - 1748-3387
VL - 15
SP - 690
EP - 697
JO - Nature Nanotechnology
JF - Nature Nanotechnology
IS - 8
ER -