In-Brain Multiphoton Imaging of Vaterite Cargoes Loaded with Carbon Dots

Hani Barhum*, Cormac McDonnell, Oleksii Peltek, Rudhvi Jain, Mariam Amer, David Kain, Galit Elad-Sfadia, Muhammad Athamna, Pablo Blinder, Pavel Ginzburg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Biocompatible fluorescent agents are key contributors to the theranostic paradigm by enabling real-time in vivo imaging. This study explores the optical properties of phenylenediamine carbon dots (CDs) and demonstrates their potential for fluorescence imaging in cells and brain blood vessels. The nonlinear absorption cross-section of the CDs was measured and achieved values near 50 Goeppert-Mayer (GM) units with efficient excitation in the 775-895 nm spectral range. Mesoporous vaterite nanoparticles were loaded with CDs to examine the possibility of a biocompatible imaging platform. Efficient one- and two-photon imaging of the CD-vaterite composites uptaken by diverse cells was demonstrated. For an in vivo scenario, CD-vaterite composites were injected into the bloodstream of a mouse, and their flow was monitored within the blood vessels of the brain through a cranial window. These results show the potential of the platform for high-brightness biocompatible imaging with the potential for both sensing and simultaneous drug delivery.

Original languageEnglish
JournalNano Letters
DOIs
StateAccepted/In press - 2024

Funding

FundersFunder number
Naomi Foundation
QuanTAU
Tel Aviv University
European Research Council802279
European Research Council
H2020 European Research Council101066138
H2020 European Research Council
Israel Science Foundation2342/21
Israel Science Foundation
Israeli Ministry of Innovation Scienc and Technology0006001
Ministry of Science, Technology and Space79518
Ministry of Science, Technology and Space

    Keywords

    • Bioimaging
    • Carbon Dots
    • Cross-section
    • Phenylenediamine
    • Two-photon fluorescence (2PF)
    • Vaterite

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