Improved signal recovery for flow cytometry based on 'spatially modulated emission'

S. Quint, J. Wittek, P. Spang, N. Levanon, T. Walther, M. Baßler

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, the technique of 'spatially modulated emission' has been introduced (Baßler et al 2008 US Patent 0080181827A1; Kiesel et al 2009 Appl. Phys. Lett. 94 041107; Kiesel et al 2011 Cytometry A79A 317-24) improving the signal-to-noise ratio (SNR) for detecting bio-particles in the field of flow cytometry. Based on this concept, we developed two advanced signal processing methods which further enhance the SNR and selectivity for cell detection. The improvements are achieved by adapting digital filtering methods from RADAR technology and mainly address inherent offset elimination, increased signal dynamics and moreover reduction of erroneous detections due to processing artifacts. Wepresent a comprehensive theory on SNR gain and provide experimental results of our concepts.

Original languageEnglish
Article number035002
JournalMethods and Applications in Fluorescence
Volume5
Issue number3
DOIs
StatePublished - 29 Aug 2017

Keywords

  • Digital filtering
  • Flow cytometry
  • LABS
  • Pulse compression
  • RADAR
  • SNR gain
  • Spatially modulated emission

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