Improved signal recovery for flow cytometry based on 'spatially modulated emission'

S. Quint; J. Wittek; P. Spang; N. Levanon; T. Walther; M. Baßler

doi:10.1088/2050-6120/aa7916

Improved signal recovery for flow cytometry based on 'spatially modulated emission'

S. Quint^*, J. Wittek, P. Spang, N. Levanon, T. Walther, M. Baßler

^*Corresponding author for this work

Department of Electrical Engineering - Systems

Research output: Contribution to journal › Article › peer-review

Abstract

Recently, the technique of 'spatially modulated emission' has been introduced (Baßler et al 2008 US Patent 0080181827A1; Kiesel et al 2009 Appl. Phys. Lett. 94 041107; Kiesel et al 2011 Cytometry A79A 317-24) improving the signal-to-noise ratio (SNR) for detecting bio-particles in the field of flow cytometry. Based on this concept, we developed two advanced signal processing methods which further enhance the SNR and selectivity for cell detection. The improvements are achieved by adapting digital filtering methods from RADAR technology and mainly address inherent offset elimination, increased signal dynamics and moreover reduction of erroneous detections due to processing artifacts. Wepresent a comprehensive theory on SNR gain and provide experimental results of our concepts.

Original language	English
Article number	035002
Journal	Methods and Applications in Fluorescence
Volume	5
Issue number	3
DOIs	https://doi.org/10.1088/2050-6120/aa7916
State	Published - 29 Aug 2017

Keywords

Digital filtering
Flow cytometry
LABS
Pulse compression
RADAR
SNR gain
Spatially modulated emission

Access to Document

10.1088/2050-6120/aa7916

Cite this

@article{c232bb587b074778986765fea0831cd0,

title = "Improved signal recovery for flow cytometry based on 'spatially modulated emission'",

abstract = "Recently, the technique of 'spatially modulated emission' has been introduced (Ba{\ss}ler et al 2008 US Patent 0080181827A1; Kiesel et al 2009 Appl. Phys. Lett. 94 041107; Kiesel et al 2011 Cytometry A79A 317-24) improving the signal-to-noise ratio (SNR) for detecting bio-particles in the field of flow cytometry. Based on this concept, we developed two advanced signal processing methods which further enhance the SNR and selectivity for cell detection. The improvements are achieved by adapting digital filtering methods from RADAR technology and mainly address inherent offset elimination, increased signal dynamics and moreover reduction of erroneous detections due to processing artifacts. Wepresent a comprehensive theory on SNR gain and provide experimental results of our concepts.",

keywords = "Digital filtering, Flow cytometry, LABS, Pulse compression, RADAR, SNR gain, Spatially modulated emission",

author = "S. Quint and J. Wittek and P. Spang and N. Levanon and T. Walther and M. Ba{\ss}ler",

note = "Publisher Copyright: {\textcopyright} 2017 IOP Publishing Ltd.",

year = "2017",

month = aug,

day = "29",

doi = "10.1088/2050-6120/aa7916",

language = "אנגלית",

volume = "5",

journal = "Methods and Applications in Fluorescence",

issn = "2050-6120",

publisher = "IOP Publishing Ltd.",

number = "3",

}

TY - JOUR

T1 - Improved signal recovery for flow cytometry based on 'spatially modulated emission'

AU - Quint, S.

AU - Wittek, J.

AU - Spang, P.

AU - Levanon, N.

AU - Walther, T.

AU - Baßler, M.

PY - 2017/8/29

Y1 - 2017/8/29

N2 - Recently, the technique of 'spatially modulated emission' has been introduced (Baßler et al 2008 US Patent 0080181827A1; Kiesel et al 2009 Appl. Phys. Lett. 94 041107; Kiesel et al 2011 Cytometry A79A 317-24) improving the signal-to-noise ratio (SNR) for detecting bio-particles in the field of flow cytometry. Based on this concept, we developed two advanced signal processing methods which further enhance the SNR and selectivity for cell detection. The improvements are achieved by adapting digital filtering methods from RADAR technology and mainly address inherent offset elimination, increased signal dynamics and moreover reduction of erroneous detections due to processing artifacts. Wepresent a comprehensive theory on SNR gain and provide experimental results of our concepts.

AB - Recently, the technique of 'spatially modulated emission' has been introduced (Baßler et al 2008 US Patent 0080181827A1; Kiesel et al 2009 Appl. Phys. Lett. 94 041107; Kiesel et al 2011 Cytometry A79A 317-24) improving the signal-to-noise ratio (SNR) for detecting bio-particles in the field of flow cytometry. Based on this concept, we developed two advanced signal processing methods which further enhance the SNR and selectivity for cell detection. The improvements are achieved by adapting digital filtering methods from RADAR technology and mainly address inherent offset elimination, increased signal dynamics and moreover reduction of erroneous detections due to processing artifacts. Wepresent a comprehensive theory on SNR gain and provide experimental results of our concepts.

KW - Digital filtering

KW - Flow cytometry

KW - LABS

KW - Pulse compression

KW - RADAR

KW - SNR gain

KW - Spatially modulated emission

UR - http://www.scopus.com/inward/record.url?scp=85044990785&partnerID=8YFLogxK

U2 - 10.1088/2050-6120/aa7916

DO - 10.1088/2050-6120/aa7916

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:85044990785

SN - 2050-6120

VL - 5

JO - Methods and Applications in Fluorescence

JF - Methods and Applications in Fluorescence

IS - 3

M1 - 035002

ER -

Improved signal recovery for flow cytometry based on 'spatially modulated emission'

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this