Improved measurement of transketolase activity in the assessment of 'TPP effect'

R. Doolman, A. Dinbar, B. A. Sela*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The indirect estimation of thiamine levels in human blood by measuring thiamine pyrophosphate effect on erythrocyte transketolase activity is the method of choice in most clinical laboratories. We describe here an optimized, time-saving, and accurate method to determine the thiamine pyrophosphate effect in as many as 16 blood samples simultaneously. The method is based on a multi-point determination using a computer remote-controlled microplate reader. For multiple sample handling, three pooled reaction mixtures are freshly prepared and loaded onto a 96 well microtitre plate. A pre-written software is then initiated to remote-control the system. The data is retrieved and processed to calculate thiamine pyrophosphate effect by a self-written 'macro' on a 'Quattro-Pro' worksheet database. This method proves to be highly accurate (coefficient of variance: 2.7%), reproducible (coefficient of variance: 4.1%) and economical.

Original languageEnglish
Pages (from-to)445-446
Number of pages2
JournalEuropean Journal of Clinical Chemistry and Clinical Biochemistry
Volume33
Issue number7
StatePublished - 1995
Externally publishedYes

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