Importance of Phenotypic and Molecular Characterization for Identification of a Neuroepithelioma Tumor Cell Line, NUB-20

Herman Yeger, Orna Mor, Gladys Pawlin, Chaim Kaplinsky, Yosef Shiloh

Research output: Contribution to journalArticlepeer-review

Abstract

A neuroblastic-like cell line (NUB-20) was derived from a case of histopathologically diagnosed metastatic neuroblastoma. The metastatic tumor and nude mouse heterotransplant resembled neuroblastoma by histological criteria, in contrast to the primary tumor, which was differentially classified as Ewing's sarcoma. However, the cell line demonstrated a unique phenotype in culture with respect to morphology, im-munohistochemical markers, and sensitivity to a battery of differentiation modulators. These characteristics, together with the presence of a chromosomal translocation (H;22),(q24;ql2) and amplification with enhanced expression of the c-myc protooncogene rather than N-myc, established this tumor as neuroepithelioma. Neuroepithelioma is a tumor type distinct from, but related to, neuroblastoma in its development from the neural crest lineage. These results emphasize the growing importance of cytogenetic and molecular markers in the classification and characterization of human tumors.

Original languageEnglish
Pages (from-to)2794-2802
Number of pages9
JournalCancer Research
Volume50
Issue number9
StatePublished - 1 May 1990

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