Impact of the Cultivation Technique on the Production of Secondary Metabolites by Chrysosporium lobatum TM-237-S5, Isolated from the Sponge Acanthella cavernosa

Géraldine Le Goff*, Philippe Lopes, Guillaume Arcile, Pinelopi Vlachou, Elsa Van Elslande, Pascal Retailleau, Jean François Gallard, Michal Weis, Yehuda Benayahu, Nikolas Fokialakis, Jamal Ouazzani

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The fungi Chrysosporium lobatum TM-237-S5 was isolated from the sponge Acanthella cavernosa, collected from the mesophotic coral ecosystem of the Red Sea. The strain was cultivated on a potato dextrose agar (PDA) medium, coupling solid-state fermentation and solid-state extraction (SSF/SSE) with a neutral macroreticular polymeric adsorbent XAD Amberlite resin (AMBERLITE XAD1600N). The SSF/SSE lead to high chemodiversity and productivity compared to classical submerged cultivation. Ten phenalenone related compounds were isolated and fully characterized by one-dimensional and two-dimensional NMR and HRMS. Among them, four were found to be new compounds corresponding to isoconiolactone, (-)-peniciphenalenin F, (+)-8-hydroxyscleroderodin, and (+)-8-hydroxysclerodin. It is concluded that SSF/SSE is a powerful strategy, opening a new era for the exploitation of microbial secondary metabolites.

Original languageEnglish
Article number678
JournalMarine Drugs
Volume17
Issue number12
DOIs
StatePublished - 2019

Funding

FundersFunder number
IUI
Interuniversity Institute for Marine Sciences in Eilat
Israel Nature and National Parks Protection Authority
Horizon 2020 Framework Programme634674
European Commission

    Keywords

    • Chrysosporium lobatum
    • Marine fungi
    • Phenalenone derivatives
    • Solid-state extraction
    • Solid-state fermentation

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