Immunosuppressive activity in culture media containing human oocytes fertilized in vitro

H. Pinkas, B. Fisch, Y. Tadir, J. Ovadia, S. Amit, B. Shohat

Research output: Contribution to journalArticlepeer-review

Abstract

A possible cause for implantation failure following embryo transfer is the rejection of pre-embryos by the maternal immune system. To elucidate the mechanisms underlying the rescue of the pre-embryo from maternal rejection, immunosuppressive activity was investigated in culture media containing human oocytes fertilized in vitro by employing the graft-versus-host reaction and the active rosette test. Normal donor lymphocytes were incubated with culture medium containing either fertilized or nonfertilized oocytes. Control experiments were carried out using lymphocytes incubated in culture medium only, as well as in culture medium containing human sperm. In 67% of the tests (12 out of 18), graft-versus-host reaction was inhibited, as compared to 29% of those performed with medium in which oocytes failed to fertilize (p < 03), and 33% with medium in which only sperm was present. The frequency of an inhibitory active rosette test was similar in both groups. It is possible that immunosuppressive properties are acquired by the human pre-embryo as it grows in culture. Detection of such substances, which could contribute to the establishment of pregnancy, may improve monitoring of embryo quality prior to transfer.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalSystems Biology in Reproductive Medicine
Volume28
Issue number1
DOIs
StatePublished - 1992

Keywords

  • IVF
  • Immunosuppressive factors
  • Pre-embryo

Fingerprint

Dive into the research topics of 'Immunosuppressive activity in culture media containing human oocytes fertilized in vitro'. Together they form a unique fingerprint.

Cite this