Immunosensing by luminescence reduction in surface-modified microstructured SU-8

Pinkie Jacob Eravuchira, Malgorzata Baranowska, Chris Eckstein, Francesc Díaz, Eduard Llobet, Lluis F. Marsal, Josep Ferré-Borrull*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


SU-8, an epoxy based negative photoresist is extensively used as a structural material for the fabrication of microelectro-mechanical systems and in microelectronics technology. However, the possible applications of SU-8 for biosensing have not been explored much, mainly because of the photoluminescence SU-8 possesses in the near-UV and visible wavelength ranges which hinders fluorescent labelling of biorecognition events. In this study we demonstrate that photoluminescence of SU-8 can be employed itself as a sensing transduction parameter to produce a tool for immunosensing: the photoluminescence shows a systematic reduction upon modification of its surface chemistry, and in particular upon attachment of an antigen-antibody (aIgG-IgG) pair. We investigate the relation of the amount of reduction of photoluminescence on planar and microstructured surfaces, and we show that microstructuring leads to a higher reduction than a planar surface. Furthermore, we evaluated the dependence of photoluminescence reduction as a function of analyte concentration to prove that this magnitude can be applied to immunosensing.

Original languageEnglish
Pages (from-to)883-888
Number of pages6
JournalApplied Surface Science
StatePublished - 15 Jan 2017
Externally publishedYes


  • Immunosensing
  • Photolithography
  • Photoluminescence
  • SU-8


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