Immunomodulatory activity of ketamine in human astroglial A172 cells: Possible relevance to its rapid antidepressant activity

Yael Yuhas, Shai Ashkenazi, Eva Berent, Abraham Weizman

Research output: Contribution to journalArticlepeer-review

Abstract

To determine if the immunomodulatory effect of ketamine is relevant to its rapid antidepressant activity, cultured human astroglial cells were incubated with ketamine, cytokine mix, or both. At 24. h, ketamine dose-dependently (100-500. μM) decreased IL-6 and TNFα production and gene expression and, at clinically relevant concentration (100. μM), augmented IL-β release and gene expression in both unstimulated and cytokine-stimulated cells. In unstimulated cells, ketamine also increased IL-8 production and mRNA expression. The reduction in IL-6 mRNA was significant within 1. h in unstimulated cells and at 4. h after stimulation. Ketamine suppressed the production of the only established depression-relevant proinflammatory cytokines, IL-6 and TNFα.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalJournal of Neuroimmunology
Volume282
DOIs
StatePublished - 15 May 2015

Keywords

  • Antidepressant
  • Astroglial cells
  • Cytokines
  • Immunomodulation
  • Ketamine

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