Immunomodulation of experimental pulmonary fibrosis by intravenous immunoglobulin (IVIG)

Vered Molina, Saleem Haj-Yahia, Inna Solodeev, Yair Levy, Miri Blank, Yehuda Shoenfeld

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To assess the immunomodulatory effect of intravenous immunoglobulin (IVIG) using an experimental model of bleomycin-induced pulmonary fibrosis. Methods: Pulmonary fibrosis was induced in C57BL/6 mice by direct intratracheal injection of bleomycin. Mice were treated with IVIG 1 week prior to (prevention protocol), or 10 days following bleomycin injection, when the disease was in progress. The controls used in the study included mice given phosphate buffered saline (PBS) and mice subjected to a commercial individual-IgG. Collagen-I deposits in the affected lungs were detected by Sirius red staining of paraffin embedded lung sections. The collagen-I content was measured by employing the hydroxyproline assay. Results : Prevention of bleomycin-induced pulmonary fibrosis by IVIG has been demonstrated by reduced expression of collagen-I protein in the affected lungs. The hydroxyproline levels in the lungs of the IVIG-treated mice were 214.33 ± 13.56 μg/1 g tissue, compared to the higher levels in lungs of IgG treated mice (342.44 ± 35.60 μg/1 g tissue) or untreated controls 328.00 ± 45.55 μg/1 g tissue, (p < 0.0001). Effective treatment of bleomycin-induced pulmonary fibrosis by IVIG has been demonstrated by the reduced expression of collagen-I protein in the affected lungs, detected by sirius red histological staining. The hydroxyproline levels in the lungs of the IVIG-treated mice were 261.00 ± 18.81 μg/1 g tissue, in comparison to the higher levels in the lungs of the IgG treated mice (342.43 ± 32.89 μg/1 g tissue) and of untreated controls (344.33 ± 49.85 μg/1 g tissue), (p < 0.001). Conclusions: Based on these preliminary studies, we conclude that IVIG may have a beneficial effect in the down regulation of collagen-I levels in the lungs of mice with bleomycin-induced pulmonary fibrosis.

Original languageEnglish
Pages (from-to)711-717
Number of pages7
JournalAutoimmunity
Volume39
Issue number8
DOIs
StatePublished - Dec 2006

Keywords

  • Animal model
  • Collagen-I
  • IVIG
  • Pulmonary fibrosis
  • TGFβ1

Fingerprint

Dive into the research topics of 'Immunomodulation of experimental pulmonary fibrosis by intravenous immunoglobulin (IVIG)'. Together they form a unique fingerprint.

Cite this