Immunological evidence for phenobarbital-stimulated synthesis of cytochrome P-450 in primary cultures of chick embryo hepatocytes

Lea Oron*, Shoshana Bar-Nun

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The induction of cytochrome P-450 by phenobarbital was studied in primary cultures of chick embryo hepatocytes. The rate of the de novo synthesis of the induced form of cytochrome P-450 was measured directly and specificially, using form-specific anti-cytochrome antibodies that quantitatively immunoprecipitated this form from the radiolabeled hepatocytes. Additionally, the steady-state levels of the cytochrome were estimated spectrophotometrically and electrophoretically. In the presence of phenobarbital the synthesis of cytochrome P-450PB by cultured hepatocytes was markedly accelerated. Furthermore, the same cytochrome P-450PB form was induced by phenobarbital in vivo in chicken liver and in the cultured chick embryo hepatocytes. Their identity was judged from immunological and electrophoretic properties of these induced cytochromes. Immunological cross-reactivity was also detected between the cytochrome P-450PB forms from chick embryo hepatocytes and from adult rat liver. The immunological cross-reactivity observed between the phenobarbital-induced cytochrome P-450 forms from different species was not observed between the different cytochrome forms with the same liver (Thomas, P.E., Reik, L.M., Ryan, D.E. and Levin, W. (1981) J. Biol. Chem. 256, 1044-1052). Implications as to the evolutionary origin of the different cytochrome forms are discussed.

Original languageEnglish
Pages (from-to)291-299
Number of pages9
JournalBBA - Molecular Cell Research
Issue number3
StatePublished - 13 Nov 1984


FundersFunder number
Israeli Academy of Science


    • (Chick embryo hepatocyte)
    • Cytochrome P-450 synthesis
    • Phenobarbital stimulation


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