Immunological and isoelectric focusing study of β-glucocerebrosidase from normal and Gaucher disease

Britta Hardy, Judith Hoffman, Z. Ossimi

Research output: Contribution to journalArticlepeer-review

Abstract

Comparison of normal and Gaucher disease β-glucocerebrosidase by agarose isoelectric focusing (IEF) demonstrated additional bands at the pI-6 area seen within the mutated enzyme, while both normal placenta and spleen enzyme preparations manifest only major activity at pI-5. Antiglucocerebrosidase antibodies precipitated both normal and pathological enzymes, however, more antibodies were needed to reach an equivalence with the normal enzymes than with the Gaucher's. Cross reactivity of the IEF isozymes were detected by direct immunodifusion on the prefocused gel.

Original languageEnglish
Pages (from-to)325-332
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume120
Issue number2
DOIs
StatePublished - 30 Apr 1984
Externally publishedYes

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