TY - JOUR
T1 - Immunological and isoelectric focusing study of β-glucocerebrosidase from normal and Gaucher disease
AU - Hardy, Britta
AU - Hoffman, Judith
AU - Ossimi, Z.
PY - 1984/4/30
Y1 - 1984/4/30
N2 - Comparison of normal and Gaucher disease β-glucocerebrosidase by agarose isoelectric focusing (IEF) demonstrated additional bands at the pI-6 area seen within the mutated enzyme, while both normal placenta and spleen enzyme preparations manifest only major activity at pI-5. Antiglucocerebrosidase antibodies precipitated both normal and pathological enzymes, however, more antibodies were needed to reach an equivalence with the normal enzymes than with the Gaucher's. Cross reactivity of the IEF isozymes were detected by direct immunodifusion on the prefocused gel.
AB - Comparison of normal and Gaucher disease β-glucocerebrosidase by agarose isoelectric focusing (IEF) demonstrated additional bands at the pI-6 area seen within the mutated enzyme, while both normal placenta and spleen enzyme preparations manifest only major activity at pI-5. Antiglucocerebrosidase antibodies precipitated both normal and pathological enzymes, however, more antibodies were needed to reach an equivalence with the normal enzymes than with the Gaucher's. Cross reactivity of the IEF isozymes were detected by direct immunodifusion on the prefocused gel.
UR - http://www.scopus.com/inward/record.url?scp=0021340736&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(84)91257-9
DO - 10.1016/0006-291X(84)91257-9
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C2 - 6375656
AN - SCOPUS:0021340736
SN - 0006-291X
VL - 120
SP - 325
EP - 332
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -