Immunohistochemical features of 3,3′,4,4′- Tetrachloroazobenzene-induced rat gingival lesions

Yuval Ramot, Marilena Vered, David E. Malarkey, Michelle J. Hooth, J. Todd Painter, Dan Dayan, Natasha Clayton, Tiwanda Masinde, Abraham Nyska*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Gingival lesions of squamous hyperplasia, cystic keratinizing hyperplasia (CKH), and squamous cell carcinoma (SCC) can be induced in rats treated by chronic gavage with 10-100 mg/kg 3,3′,4,4′-tetrachloroazobenzene. We evaluated gingival squamous hyperplasia (GSH), CKH, and SCC for the immunohistochemical pattern of expression of carcinogenesis-associated markers. The 3 types of lesions and controls were stained with proliferation markers (proliferating cell nuclear antigen [PCNA] and cyclin-D1), tumor-suppressor markers (β-catenin and mammary serine protease inhibitor [maspin]) and stroma-related markers (α-smooth muscle actin [SMA] and osteonectin/SPARC). The lesions had common immunohistochemical characteristics that differed in their expression patterns among the various diagnoses. PCNA and cyclin-D1 expression was higher in GSH, CKH, and SCC than in controls. The normal membranous expression of β-catenin was lower in GSH, and almost absent in CKH and SCC. Maspin expression was similar in GSH and controls, whereas both CKH and SCC showed decreased expression. SMA and/or osteonectin/SPARC were seen in stromal cells in CKH and SCC. Collectively, there appears to be a progression from hyperplastic and cystic lesions toward malignancy based on the morphological changes, supported by the expression of carcinogenesis-associated proteins. The exact sequence of events leading to SCC remains to be defined in a time-dependent manner.

Original languageEnglish
Pages (from-to)577-592
Number of pages16
JournalToxicologic Pathology
Volume40
Issue number4
DOIs
StatePublished - Jun 2012

Keywords

  • TCAB
  • cystic keratinizing hyperplasia
  • gingival squamous hyperplasia
  • rat
  • squamous cell carcinoma

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