TY - JOUR
T1 - Immunoglobulin heavy chain gene rearrangements in chronic lymphocytic leukaemia
T2 - correlation with clinical stage
AU - Rechavi, Gideon
AU - Mandel, Mathilda
AU - Katzir, Nurit
AU - Brok‐Simoni, Frida
AU - Hakim, Itzhak
AU - Holtzman, Fanny
AU - Biniaminov, Miriam
AU - Givol, David
AU - Ben‐Bassat, Isaac
AU - Ramot, Bracha
PY - 1989/8
Y1 - 1989/8
N2 - Summary A search for a correlation between the clinical stage of chronic lymphocytic leukaemia (CLL) and the pattern of immunoglobulin heavy chain gene rearrangements was undertaken. DNA samples from the leukaemic cells of 38 CLL patients were analysed by Southern blot hybridization. Using probes for the immunoglobulin heavy chain J (JH) and Cμ regions a marked heterogeneity of the hybridization patterns was observed in both regions. The number of JH hybridization bands varied from one to four and more than two were found in 58% of the patients. In 42% of the patients no germline JH genes were found. One to three additional Cμ bands were observed in 34%, but the germline was preserved in all samples. There was no correlation between the clinical stage and the number of hybridizing JH bands; however, a significant correlation was found between the loss of JH germline band or a Cμ multiband pattern and advanced stage of the disease. The genetic events in the immunoglobulin genes observed in advanced CLL patients are assumed to result from clonal evolution and tumour progression.
AB - Summary A search for a correlation between the clinical stage of chronic lymphocytic leukaemia (CLL) and the pattern of immunoglobulin heavy chain gene rearrangements was undertaken. DNA samples from the leukaemic cells of 38 CLL patients were analysed by Southern blot hybridization. Using probes for the immunoglobulin heavy chain J (JH) and Cμ regions a marked heterogeneity of the hybridization patterns was observed in both regions. The number of JH hybridization bands varied from one to four and more than two were found in 58% of the patients. In 42% of the patients no germline JH genes were found. One to three additional Cμ bands were observed in 34%, but the germline was preserved in all samples. There was no correlation between the clinical stage and the number of hybridizing JH bands; however, a significant correlation was found between the loss of JH germline band or a Cμ multiband pattern and advanced stage of the disease. The genetic events in the immunoglobulin genes observed in advanced CLL patients are assumed to result from clonal evolution and tumour progression.
UR - http://www.scopus.com/inward/record.url?scp=0024458106&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2141.1989.tb04317.x
DO - 10.1111/j.1365-2141.1989.tb04317.x
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C2 - 2505833
AN - SCOPUS:0024458106
SN - 0007-1048
VL - 72
SP - 524
EP - 529
JO - British Journal of Haematology
JF - British Journal of Haematology
IS - 4
ER -