Immunogenicity in calves of a crude ribosomal fraction of Trichophyton verrucosum: A field trial

D. Elad, E. Segal*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


This paper describes a field trial involving 13 calves raised on a farm known to be endemic for Trichophyton verrucosum infection. Seven calves were immunized by subcutaneous inoculations with a crude ribosomal fraction (CRF) of T. verrucosum suspended in aluminium hydroxide as adjuvant. Six animals were sham-immunized with buffer suspended in the adjuvant and served as controls. Two injections were given: one at the age of 2 weeks and a second 2 weeks later. CRF was prepared from T. verrucosum cultures grown in a vitamin-enriched liquid medium. The fungal mat was disrupted mechanically and CRF was separated from the cell-free extract by differential ultracentrifugation. The CRF was characterized biochemically (RNA and protein content) and physically (electron microscopy). The protection induced by vaccination was assessed through a clinical follow-up of the animals to determine the presence and duration of dermatophyte infection following their exposure to a T. verrucosum-contaminated environment. Vaccination with the CRF resulted in a statistically significant decrease in the period during which clinical signs of dermatophytosis were observed (from a mean of 9.5 weeks to 3.7 weeks). To assess the humoral response, serum samples were taken before each vaccination and 2 weeks after the second inoculation. For the cell-mediated immune (CMI) response assessment, whole-blood samples were taken 2 weeks after the second vaccination. The presence of anti-T. verrucosum antibodies in the sera was determined by ELISA and the CMI response was assessed in vitro by the lymphocyte stimulation test. The results indicate that both the cell-mediated and humoral components of the immune system were stimulated by vaccination. The overall results of the present research indicate that the T. verrucosum CRF shows potential as a basis for the development of a vaccine against bovine tricophytosis.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
Issue number1
StatePublished - Jan 1995


  • Calves: Trichophyton verrucosum
  • ribosomes
  • vaccine


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