Abstract
Objective: To investigate the immunocytochemical expression and presence of mRNA transcripts of oncostatin M (OSM) and its exclusive receptor (OSM-Rβ) in ovaries from human adults and fetuses. Design: Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study. Setting: Major tertiary care and referral academic centers. Patient(s): Ten women and girls undergoing laparoscopic ovarian biopsy and 30 women undergoing second-trimester and third-trimester pregnancy terminations. Intervention(s): None. Main Outcome Measure(s): Microscopic morphometric analysis, immunocytochemistry for OSM and OSM-Rβ, and RT-PCR analyses. Result(s): There was strong to moderate immunocytochemical staining for OSM in both oocytes and granulosa cells of follicles from primordial stages onwards in ovaries from both fetuses and adults/adolescents. OSM-Rβ was detected mainly in the oocytes. Transcripts of OSM and OSM-Rβ RNA were detected by RT-PCR analyses. Conclusion(s): The expression of OSM and its receptor in ovarian tissue from fetuses and women suggests a possible role of OSM in growth initiation of human primordial follicles.
Original language | English |
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Pages (from-to) | 1188-1196 |
Number of pages | 9 |
Journal | Fertility and Sterility |
Volume | 83 |
Issue number | 4 SUPPL. |
DOIs | |
State | Published - Apr 2005 |
Keywords
- Immunocytochemistry
- OSM
- OSM-Rβ
- PGC
- Primordial follicles
- gp 130
- mRNA