@article{2e47373fd0f6453d9ac60f2b6c89162c,
title = "Immunocytochemical detection and reverse transcription polymerase chain reaction expression of oncostatin M (OSM) and its receptor (OSM-Rβ) in human fetal and adult ovaries",
abstract = "Objective: To investigate the immunocytochemical expression and presence of mRNA transcripts of oncostatin M (OSM) and its exclusive receptor (OSM-Rβ) in ovaries from human adults and fetuses. Design: Immunocytochemical and reverse transcription polymerase chain reaction (RT-PCR) study. Setting: Major tertiary care and referral academic centers. Patient(s): Ten women and girls undergoing laparoscopic ovarian biopsy and 30 women undergoing second-trimester and third-trimester pregnancy terminations. Intervention(s): None. Main Outcome Measure(s): Microscopic morphometric analysis, immunocytochemistry for OSM and OSM-Rβ, and RT-PCR analyses. Result(s): There was strong to moderate immunocytochemical staining for OSM in both oocytes and granulosa cells of follicles from primordial stages onwards in ovaries from both fetuses and adults/adolescents. OSM-Rβ was detected mainly in the oocytes. Transcripts of OSM and OSM-Rβ RNA were detected by RT-PCR analyses. Conclusion(s): The expression of OSM and its receptor in ovarian tissue from fetuses and women suggests a possible role of OSM in growth initiation of human primordial follicles.",
keywords = "Immunocytochemistry, OSM, OSM-Rβ, PGC, Primordial follicles, gp 130, mRNA",
author = "Ronit Abir and Asangla Ao and Shaoguang Jin and Miriam Barnett and {Van Den Hurk}, Robert and Sarit Freimann and Benjamin Fisch",
note = "Funding Information: This work was partially supported by research grants from the Israel Cancer Association. ",
year = "2005",
month = apr,
doi = "10.1016/j.fertnstert.2004.10.043",
language = "אנגלית",
volume = "83",
pages = "1188--1196",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "4 SUPPL.",
}