Immunochemical Visualization of Binding of the Chemical Carcinogen Benzo(a)pyrene Diol-Epoxide 1 to the Genome

Hanoch Slor; Hiroshi Mizusawa; Natasha Neiharti; Tsuyoshi Kakefuda; Rufus S. Day; Michael Bustin

Immunochemical Visualization of Binding of the Chemical Carcinogen Benzo(a)pyrene Diol-Epoxide 1 to the Genome

Hanoch Slor, Hiroshi Mizusawa, Natasha Neiharti, Tsuyoshi Kakefuda, Rufus S. Day, Michael Bustin

National Institutes of Health

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Antisera against DNA modified with r-7, t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE-1) was elicited in rabbits. Such sera reacted with either single or double-stranded modified DNA but not with unmodified DNA, free benzo(a)pyrene, or proteins modified by BPDE-1. Indirect immunofluorescence studies indicated that the immunoglobulin G in the sera bound specifically to the nuclei of KD cells which were treated with BPDE-1. The intensity of fluorescence was proportional to the dose of BPDE-1 used to treat the cells. About 50% of the BPDE-1-DNA adducts remained bound to DNA 24 hr after the removal of the carcinogen. The location of BPDE-1-modified bases in Col E1 DNA was visualized by immunoelectron microscopy.

Original language	English
Pages (from-to)	3111-3117
Number of pages	7
Journal	Cancer Research
Volume	41
Issue number	8
State	Published - 1 Aug 1981
Externally published	Yes

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title = "Immunochemical Visualization of Binding of the Chemical Carcinogen Benzo(a)pyrene Diol-Epoxide 1 to the Genome",

abstract = "Antisera against DNA modified with r-7, t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE-1) was elicited in rabbits. Such sera reacted with either single or double-stranded modified DNA but not with unmodified DNA, free benzo(a)pyrene, or proteins modified by BPDE-1. Indirect immunofluorescence studies indicated that the immunoglobulin G in the sera bound specifically to the nuclei of KD cells which were treated with BPDE-1. The intensity of fluorescence was proportional to the dose of BPDE-1 used to treat the cells. About 50% of the BPDE-1-DNA adducts remained bound to DNA 24 hr after the removal of the carcinogen. The location of BPDE-1-modified bases in Col E1 DNA was visualized by immunoelectron microscopy.",

author = "Hanoch Slor and Hiroshi Mizusawa and Natasha Neiharti and Tsuyoshi Kakefuda and Day, {Rufus S.} and Michael Bustin",

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AU - Slor, Hanoch

AU - Mizusawa, Hiroshi

AU - Neiharti, Natasha

AU - Kakefuda, Tsuyoshi

AU - Day, Rufus S.

AU - Bustin, Michael

PY - 1981/8/1

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AB - Antisera against DNA modified with r-7, t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE-1) was elicited in rabbits. Such sera reacted with either single or double-stranded modified DNA but not with unmodified DNA, free benzo(a)pyrene, or proteins modified by BPDE-1. Indirect immunofluorescence studies indicated that the immunoglobulin G in the sera bound specifically to the nuclei of KD cells which were treated with BPDE-1. The intensity of fluorescence was proportional to the dose of BPDE-1 used to treat the cells. About 50% of the BPDE-1-DNA adducts remained bound to DNA 24 hr after the removal of the carcinogen. The location of BPDE-1-modified bases in Col E1 DNA was visualized by immunoelectron microscopy.

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Immunochemical Visualization of Binding of the Chemical Carcinogen Benzo(a)pyrene Diol-Epoxide 1 to the Genome

Abstract

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