Immune co-culture cell microarray–a feasible tool for high-throughput functional investigation of lymphocyte–cancer interactions

Erez Nissim Baruch, Rona Ortenberg, Camila Avivi, Liat Anafi, Daniela Dick-Necula, Chani Stossel, Yonatan Moshkovits, Orit Itzhaki, Michal Judith Besser, Jacob Schachter, Iris Barshack, Gal Markel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Omics analyses often result in dozens to hundreds of potential targets, requiring validation for their biological relevance. Current high-throughput functional investigation methods are frequently labor-intensive, expensive, and display low reproducibility. The Immune Co-Culture Cell Microarray (ICCM) is a formalin-fixed paraffin-embedded cell block microarray based on co-cultures of patient-derived tumor-infiltrating lymphocytes and their autologous melanoma cells. Each ICCM slide represents the same experiment and can be stained using standard immunohistochemistry and immunofluorescence techniques. Functional dynamics assessment of both proteins and microRNAs using ICCM stained slides demonstrated similar findings to flow cytometry assays and to previously published patient-derived biopsy reports.

Original languageEnglish
Article number1741267
JournalOncoImmunology
Volume9
Issue number1
DOIs
StatePublished - 1 Jan 2020

Keywords

  • Immunotherapy
  • functional protein expression
  • immunological cytotoxicity test
  • melanoma
  • omics validation

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