Abstract
The incorporation of thymidine by lymphocytes stimulated by phytohemagglutinin (PHA) was correlated with the number of immature cells identified by the Technicon H-1 as blasts. The number of blasts and thymidine incorporation plateaued on day 3. At that time, a higher linear correlation between the two variables was found (r = 0.949, p<0.01). The H-1 evaluated the samples in 60 seconds as compared to 3 hours of processing required to calculate thymidine incorporation. We conclude that the number of immature cells identified by the Technicon H-1 can predict the degree of thymidine incorporation after stimulation of peripheral blood cells with PHA. The H-1 may replace the more time consuming and dangerous use of radioactive thymidine incorporation in evaluating patients' functional immunologic status.
Original language | English |
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Pages (from-to) | 601-605 |
Number of pages | 5 |
Journal | Blood Cells |
Volume | 15 |
Issue number | 3 |
State | Published - 1989 |
Externally published | Yes |