Immature cells identified by the H-1 hematology analyzer predicts thymidine incorporation after mitogenic stimulation

P. Froom, A. Kinarty, E. Aghai, N. Lahat

Research output: Contribution to journalArticlepeer-review

Abstract

The incorporation of thymidine by lymphocytes stimulated by phytohemagglutinin (PHA) was correlated with the number of immature cells identified by the Technicon H-1 as blasts. The number of blasts and thymidine incorporation plateaued on day 3. At that time, a higher linear correlation between the two variables was found (r = 0.949, p<0.01). The H-1 evaluated the samples in 60 seconds as compared to 3 hours of processing required to calculate thymidine incorporation. We conclude that the number of immature cells identified by the Technicon H-1 can predict the degree of thymidine incorporation after stimulation of peripheral blood cells with PHA. The H-1 may replace the more time consuming and dangerous use of radioactive thymidine incorporation in evaluating patients' functional immunologic status.

Original languageEnglish
Pages (from-to)601-605
Number of pages5
JournalBlood Cells
Volume15
Issue number3
StatePublished - 1989
Externally publishedYes

Fingerprint

Dive into the research topics of 'Immature cells identified by the H-1 hematology analyzer predicts thymidine incorporation after mitogenic stimulation'. Together they form a unique fingerprint.

Cite this