@article{ec220bced2714a999abc053d80f4542f,
title = "Imaging the assembly and disassembly kinetics of cis-SNARE complexes on native plasma membranes",
abstract = "Mild sonication of eukaryotic cells produces native plasma membrane sheets that retain their docked organelles, cytoskeleton structures and cytoplasmic complexes. While the delicate organization of membranous protein complexes remains undisturbed, their inner plasmalemmel leaflet can be rapidly exposed to bathing solutions, enabling specific biochemical manipulations. Here, we apply this system to track membrane-biochemistry kinetics. We monitor soluble NSF-attachment protein receptor (SNARE) complex assembly and disassembly on the plasma membrane at high time resolution. The results suggest two-phase kinetics for the assembly process and dependence of the disassembly kinetics on both N-ethyl maleimide-sensitive factor (NSF) and soluble NSF-attachment protein (α-SNAP) concentrations.",
keywords = "Assembly, Disassembly, Ex vivo, Plasma membrane sheet, Time-resolved kinetics, cis-SNARE complex",
author = "Dana Bar-On and Ulrike Winter and Esther Nachliel and Menachem Gutman and Dirk Fasshauer and Thorsten Lang and Uri Ashery",
note = "Funding Information: The authors would like to thank Professor R. Jahn for his constant support, Dr. F.E. Zilly for providing recombinant protein, and D. Diezmann for excellent technical assistance and for providing recombinant proteins. D.B.O. and U.W. were supported by the Max Planck Society and a grant from the Deutsche Forschungsgemeinschaft (SFB 523), respectively.",
year = "2008",
month = oct,
day = "15",
doi = "10.1016/j.febslet.2008.08.040",
language = "אנגלית",
volume = "582",
pages = "3563--3568",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "John Wiley and Sons Inc.",
number = "23-24",
}