Imaging of somatic Ca2+ transients in differentiated human neurons

Irena Vertkin, Dalit Ben-Yosef

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Calcium is a major regulator of neuronal activity and calcium signaling is critically important for normal neuronal function. Ca imaging is a well-established tool for studying neuronal function and ongoing spontaneous Ca2+ transients are a good indicator of neuronal maturity. There are various indicators available today, differing by their sensitivity, spectra, and loading method. Here we present a method for measurement of Ca2+ transients in neurons using two different Ca2+ indicators, Oregon Green BAPTA-1 and GCaMP6.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-129
Number of pages7
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1942
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Calcium indicators
  • GCaMP6
  • Human induced pluripotent stem cells
  • Oregon green-488 BAPTA-1AM
  • Somatic Ca transients

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