Imaging H2/17O distribution in a phantom and measurement of metabolically produced H2/17O in live mice by proton NMR

Itamar Ronen, Jing Huei Lee, Hellmut Merkle, Kamil Ugurbil, Gil Navon

Research output: Contribution to journalArticlepeer-review

Abstract

Previously we suggested a method for indirect detection of H2/17O via a combination of 1H spin-echo sequence and 17O decoupling. The method is based on the shortening of the water proton T2 due to the residual spin- spin coupling with the 17O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the 17O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H2/17O, and report for the first time an application of this method to in vivo detection of H2/17O in live newborn mice, where the H2/17O was introduced either directly i.p. or by inhalation of 17O2 where it was formed by oxidative metabolic activity.

Original languageEnglish
Pages (from-to)333-340
Number of pages8
JournalNMR in Biomedicine
Volume10
Issue number7
DOIs
StatePublished - Oct 1997

Keywords

  • H/O, O NMR
  • Indirect detection

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