Imaging H2/17O distribution in a phantom and measurement of metabolically produced H2/17O in live mice by proton NMR

Itamar Ronen; Jing Huei Lee; Hellmut Merkle; Kamil Ugurbil; Gil Navon

doi:10.1002/(SICI)1099-1492(199710)10:7<333::AID-NBM465>3.0.CO;2-E

Imaging H₂/¹⁷O distribution in a phantom and measurement of metabolically produced H₂/¹⁷O in live mice by proton NMR

Itamar Ronen, Jing Huei Lee, Hellmut Merkle, Kamil Ugurbil, Gil Navon^*

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Previously we suggested a method for indirect detection of H₂/¹⁷O via a combination of ¹H spin-echo sequence and ¹⁷O decoupling. The method is based on the shortening of the water proton T₂ due to the residual spin- spin coupling with the ¹⁷O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the ¹⁷O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H₂/¹⁷O, and report for the first time an application of this method to in vivo detection of H₂/¹⁷O in live newborn mice, where the H₂/¹⁷O was introduced either directly i.p. or by inhalation of ¹⁷O₂ where it was formed by oxidative metabolic activity.

Original language	English
Pages (from-to)	333-340
Number of pages	8
Journal	NMR in Biomedicine
Volume	10
Issue number	7
DOIs	https://doi.org/10.1002/(SICI)1099-1492(199710)10:7<333::AID-NBM465>3.0.CO;2-E
State	Published - Oct 1997

Keywords

H/O, O NMR
Indirect detection

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10.1002/(SICI)1099-1492(199710)10:7<333::AID-NBM465>3.0.CO;2-E

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title = "Imaging H2/17O distribution in a phantom and measurement of metabolically produced H2/17O in live mice by proton NMR",

abstract = "Previously we suggested a method for indirect detection of H2/17O via a combination of 1H spin-echo sequence and 17O decoupling. The method is based on the shortening of the water proton T2 due to the residual spin- spin coupling with the 17O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the 17O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H2/17O, and report for the first time an application of this method to in vivo detection of H2/17O in live newborn mice, where the H2/17O was introduced either directly i.p. or by inhalation of 17O2 where it was formed by oxidative metabolic activity.",

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author = "Itamar Ronen and Lee, {Jing Huei} and Hellmut Merkle and Kamil Ugurbil and Gil Navon",

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AU - Ronen, Itamar

AU - Lee, Jing Huei

AU - Merkle, Hellmut

AU - Ugurbil, Kamil

AU - Navon, Gil

PY - 1997/10

Y1 - 1997/10

N2 - Previously we suggested a method for indirect detection of H2/17O via a combination of 1H spin-echo sequence and 17O decoupling. The method is based on the shortening of the water proton T2 due to the residual spin- spin coupling with the 17O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the 17O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H2/17O, and report for the first time an application of this method to in vivo detection of H2/17O in live newborn mice, where the H2/17O was introduced either directly i.p. or by inhalation of 17O2 where it was formed by oxidative metabolic activity.

AB - Previously we suggested a method for indirect detection of H2/17O via a combination of 1H spin-echo sequence and 17O decoupling. The method is based on the shortening of the water proton T2 due to the residual spin- spin coupling with the 17O nucleus, and on our finding that this shortening is abolished by applying an radio frequency field at the 17O resonance frequency. In the present work we demonstrate how the method can be used for imaging low concentrations of H2/17O, and report for the first time an application of this method to in vivo detection of H2/17O in live newborn mice, where the H2/17O was introduced either directly i.p. or by inhalation of 17O2 where it was formed by oxidative metabolic activity.

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Imaging H₂/¹⁷O distribution in a phantom and measurement of metabolically produced H₂/¹⁷O in live mice by proton NMR

Abstract

Keywords

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