TY - JOUR
T1 - IGBT-Based Pulsed Electric Fields Generator for Disinfection
T2 - Design and In Vitro Studies on Pseudomonas aeruginosa
AU - Rubin, Andrey Ethan
AU - Levkov, Klimenty
AU - Usta, Osman Berk
AU - Yarmush, Martin
AU - Golberg, Alexander
N1 - Publisher Copyright:
© 2019, Biomedical Engineering Society.
PY - 2019/5/15
Y1 - 2019/5/15
N2 - Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm −1 , when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm 2 area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.
AB - Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm −1 , when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm 2 area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.
KW - Electroporation
KW - IGBT
KW - Multidrug-resistant bacteria
KW - Pseudomonas aeruginosa PAO1
KW - Pulsed electric field
UR - http://www.scopus.com/inward/record.url?scp=85061214461&partnerID=8YFLogxK
U2 - 10.1007/s10439-019-02225-0
DO - 10.1007/s10439-019-02225-0
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AN - SCOPUS:85061214461
SN - 0090-6964
VL - 47
SP - 1314
EP - 1325
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 5
ER -