IGBT-Based Pulsed Electric Fields Generator for Disinfection: Design and In Vitro Studies on Pseudomonas aeruginosa

Andrey Ethan Rubin, Klimenty Levkov, Osman Berk Usta, Martin Yarmush, Alexander Golberg

Research output: Contribution to journalArticlepeer-review

Abstract

Irreversible electroporation of cell membrane with pulsed electric fields is an emerging physical method for disinfection that aims to reduce the doses and volumes of used antibiotics for wound healing. Here we report on the design of the IGBT-based pulsed electric field generator that enabled eradication of multidrug resistant Pseudomonas aeruginosa PAO1 on the gel. Using a concentric electric configuration we determined that the lower threshold of the electric field required to kill P. aeruginosa PAO1 was 89.28 ± 12.89 V mm −1 , when 200 square pulses of 300 µs duration are delivered at 3 Hz. These parameters disinfected 38.14 ± 0.79 mm 2 area around the single needle electrode. This study provides a step towards the design of equipment required for multidrug-resistant bacteria disinfection in patients with pulsed electric fields.

Original languageEnglish
Pages (from-to)1314-1325
Number of pages12
JournalAnnals of Biomedical Engineering
Volume47
Issue number5
DOIs
StatePublished - 15 May 2019

Keywords

  • Electroporation
  • IGBT
  • Multidrug-resistant bacteria
  • Pseudomonas aeruginosa PAO1
  • Pulsed electric field

Fingerprint

Dive into the research topics of 'IGBT-Based Pulsed Electric Fields Generator for Disinfection: Design and In Vitro Studies on Pseudomonas aeruginosa'. Together they form a unique fingerprint.

Cite this