Identification of the subunit carrying FeS-centers A and B in the P840-reaction center preparation of Chlorobium limicola

Natascha Illinger; Dian Lin Xie; Günter Hauska; Nathan Nelson

doi:10.1007/BF00015067

Identification of the subunit carrying FeS-centers A and B in the P840-reaction center preparation of Chlorobium limicola

Natascha Illinger^*
, Dian Lin Xie
, Günter Hauska
, Nathan Nelson

^*Corresponding author for this work

University of Regensburg
Hoffmann-La Roche Inc.

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The product of the second gene in a transcription unit for the P840-reaction center of Chlorobium limicola f.sp. thiosulfatophilum, which codes for a protein of 23.87 kDa with 232 amino acids, was identified as the subunit migrating in SDS-PAGE at the apparent molecular weight of 32 kDa in reaction center preparations, by Western blotting and N-terminal sequencing. This protein corresponds to PsaC, a 8 kDa-subunit of Photosystem 1 which carries the FeS-centers A and B.

Original language	English
Pages (from-to)	111-114
Number of pages	4
Journal	Photosynthesis Research
Volume	38
Issue number	1
DOIs	https://doi.org/10.1007/BF00015067
State	Published - Oct 1993
Externally published	Yes

Keywords

FeS-centers A and B
Photosystem 1 reaction center
bound ferredoxin
green sulfur bacteria
psa-C

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10.1007/BF00015067

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title = "Identification of the subunit carrying FeS-centers A and B in the P840-reaction center preparation of Chlorobium limicola",

abstract = "The product of the second gene in a transcription unit for the P840-reaction center of Chlorobium limicola f.sp. thiosulfatophilum, which codes for a protein of 23.87 kDa with 232 amino acids, was identified as the subunit migrating in SDS-PAGE at the apparent molecular weight of 32 kDa in reaction center preparations, by Western blotting and N-terminal sequencing. This protein corresponds to PsaC, a 8 kDa-subunit of Photosystem 1 which carries the FeS-centers A and B.",

keywords = "FeS-centers A and B, Photosystem 1 reaction center, bound ferredoxin, green sulfur bacteria, psa-C",

author = "Natascha Illinger and Xie, \{Dian Lin\} and G{\"u}nter Hauska and Nathan Nelson",

year = "1993",

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AU - Illinger, Natascha

AU - Xie, Dian Lin

AU - Hauska, Günter

AU - Nelson, Nathan

PY - 1993/10

Y1 - 1993/10

N2 - The product of the second gene in a transcription unit for the P840-reaction center of Chlorobium limicola f.sp. thiosulfatophilum, which codes for a protein of 23.87 kDa with 232 amino acids, was identified as the subunit migrating in SDS-PAGE at the apparent molecular weight of 32 kDa in reaction center preparations, by Western blotting and N-terminal sequencing. This protein corresponds to PsaC, a 8 kDa-subunit of Photosystem 1 which carries the FeS-centers A and B.

AB - The product of the second gene in a transcription unit for the P840-reaction center of Chlorobium limicola f.sp. thiosulfatophilum, which codes for a protein of 23.87 kDa with 232 amino acids, was identified as the subunit migrating in SDS-PAGE at the apparent molecular weight of 32 kDa in reaction center preparations, by Western blotting and N-terminal sequencing. This protein corresponds to PsaC, a 8 kDa-subunit of Photosystem 1 which carries the FeS-centers A and B.

KW - FeS-centers A and B

KW - Photosystem 1 reaction center

KW - bound ferredoxin

KW - green sulfur bacteria

KW - psa-C

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JO - Photosynthesis Research

JF - Photosynthesis Research

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ER -

Identification of the subunit carrying FeS-centers A and B in the P840-reaction center preparation of Chlorobium limicola

Abstract

Keywords

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