Identification of the rhamnose-sensitive adhesin of Capnocytophaga ochracea ATCC 33596

E. I. Weiss; I. Eli; B. Shenitzki; N. Smorodinsky

doi:10.1016/0003-9969(90)90142-W

Identification of the rhamnose-sensitive adhesin of Capnocytophaga ochracea ATCC 33596

E. I. Weiss, I. Eli, B. Shenitzki, N. Smorodinsky

Research output: Contribution to journal › Article › peer-review

Abstract

The coaggregation of the Gram-negative microoganism Capnocytophaga ochracea ATCC 33596 with several Gram-positive bacteria, including streptococci, actinomyces and rothia, is mediated by rhamnose-sensitive adhesin(s). In the present study, MAbs against the C. ochracea adhesin(s) were prepared. These antibodies inhibited all the rhamnose-sensitive interactions, indicating that they recognize epitopes at or near the rhamnose binding site of the adhesin. The monoclonals served as probes in immunoblot analysis and recognized a polypeptide of M_r 155 K present in the wild-type organism but absent in a coaggregation defective mutant. It is concluded that the rhamnose-sensitive interactions of C. ochracea are mediated by a 155 K M_r polypeptide present on its outer surface.

Original language	English
Pages (from-to)	S127-S130
Journal	Archives of Oral Biology
Volume	35
Issue number	SUPPL.
DOIs	https://doi.org/10.1016/0003-9969(90)90142-W
State	Published - 1990

Keywords

Capnocytophaga ochracea
adhesin
coaggregation
monoclonal antibody
rhamnose-binding

Access to Document

10.1016/0003-9969(90)90142-W

Cite this

@article{b474924e89fb4f61890f9a1ded025e7b,

title = "Identification of the rhamnose-sensitive adhesin of Capnocytophaga ochracea ATCC 33596",

abstract = "The coaggregation of the Gram-negative microoganism Capnocytophaga ochracea ATCC 33596 with several Gram-positive bacteria, including streptococci, actinomyces and rothia, is mediated by rhamnose-sensitive adhesin(s). In the present study, MAbs against the C. ochracea adhesin(s) were prepared. These antibodies inhibited all the rhamnose-sensitive interactions, indicating that they recognize epitopes at or near the rhamnose binding site of the adhesin. The monoclonals served as probes in immunoblot analysis and recognized a polypeptide of Mr 155 K present in the wild-type organism but absent in a coaggregation defective mutant. It is concluded that the rhamnose-sensitive interactions of C. ochracea are mediated by a 155 K Mr polypeptide present on its outer surface.",

keywords = "Capnocytophaga ochracea, adhesin, coaggregation, monoclonal antibody, rhamnose-binding",

author = "Weiss, {E. I.} and I. Eli and B. Shenitzki and N. Smorodinsky",

note = "Funding Information: Acknowledgements-This researchw as carried out in the Alpha OmegaR esearchL aboratoriesa nd supportedb y the Fund for Basic ResearchG rant No. 171/88b, y the Israel Academyo f Sciencesa nd Humanitiesa nd Grant No. 880020f0ro m the UnitedS tates-IsraBeli nationaSl c_ie__n_c_e_ Foundation( BSF), Jerusalem,I srael. The editoriala ssist-anceo f Mrs Rita Lazar is greatlya ppreciated.",

year = "1990",

doi = "10.1016/0003-9969(90)90142-W",

language = "אנגלית",

volume = "35",

pages = "S127--S130",

journal = "Archives of Oral Biology",

issn = "0003-9969",

publisher = "Elsevier Ltd.",

number = "SUPPL.",

}

TY - JOUR

T1 - Identification of the rhamnose-sensitive adhesin of Capnocytophaga ochracea ATCC 33596

AU - Weiss, E. I.

AU - Eli, I.

AU - Shenitzki, B.

AU - Smorodinsky, N.

N1 - Funding Information: Acknowledgements-This researchw as carried out in the Alpha OmegaR esearchL aboratoriesa nd supportedb y the Fund for Basic ResearchG rant No. 171/88b, y the Israel Academyo f Sciencesa nd Humanitiesa nd Grant No. 880020f0ro m the UnitedS tates-IsraBeli nationaSl c_ie__n_c_e_ Foundation( BSF), Jerusalem,I srael. The editoriala ssist-anceo f Mrs Rita Lazar is greatlya ppreciated.

PY - 1990

Y1 - 1990

N2 - The coaggregation of the Gram-negative microoganism Capnocytophaga ochracea ATCC 33596 with several Gram-positive bacteria, including streptococci, actinomyces and rothia, is mediated by rhamnose-sensitive adhesin(s). In the present study, MAbs against the C. ochracea adhesin(s) were prepared. These antibodies inhibited all the rhamnose-sensitive interactions, indicating that they recognize epitopes at or near the rhamnose binding site of the adhesin. The monoclonals served as probes in immunoblot analysis and recognized a polypeptide of Mr 155 K present in the wild-type organism but absent in a coaggregation defective mutant. It is concluded that the rhamnose-sensitive interactions of C. ochracea are mediated by a 155 K Mr polypeptide present on its outer surface.

AB - The coaggregation of the Gram-negative microoganism Capnocytophaga ochracea ATCC 33596 with several Gram-positive bacteria, including streptococci, actinomyces and rothia, is mediated by rhamnose-sensitive adhesin(s). In the present study, MAbs against the C. ochracea adhesin(s) were prepared. These antibodies inhibited all the rhamnose-sensitive interactions, indicating that they recognize epitopes at or near the rhamnose binding site of the adhesin. The monoclonals served as probes in immunoblot analysis and recognized a polypeptide of Mr 155 K present in the wild-type organism but absent in a coaggregation defective mutant. It is concluded that the rhamnose-sensitive interactions of C. ochracea are mediated by a 155 K Mr polypeptide present on its outer surface.

KW - Capnocytophaga ochracea

KW - adhesin

KW - coaggregation

KW - monoclonal antibody

KW - rhamnose-binding

UR - http://www.scopus.com/inward/record.url?scp=0025654805&partnerID=8YFLogxK

U2 - 10.1016/0003-9969(90)90142-W

DO - 10.1016/0003-9969(90)90142-W

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

AN - SCOPUS:0025654805

VL - 35

SP - S127-S130

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - SUPPL.

ER -

Identification of the rhamnose-sensitive adhesin of Capnocytophaga ochracea ATCC 33596

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this