Identification of the herpes simplex virus DNA sequences present in six herpes simplex virus thymidine kinase transformed mouse cell lines

J. M. Leiden, N. Frenkel, F. Rapp

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We have used a novel filter hybridization approach to detect and map the herpes simplex virus (HSV) DNA sequences which are present in four HSV thymidine kinase (HSVtk+) transformed cell lines which were derived by exposure of thymidine kinase negative (tk-) mouse cells to UV light irradiated HSV type 2 (HSV 2). In addition, we have mapped the HSV 1 DNA sequences which are present in two HSV 1tk+ transformed cell lines produced by transfection of tk- mouse cells with sheared HSV 1 DNA. The results of these studies can be summarized as follows. (i) The only HSV DNA sequences which were common to all HSVtk+ transformed cells were those located between map coordinates 0.28 and 0.32. Thus, this region contains all the viral DNA sequences which are necessary for the expression of HSV mediated tk transformation. (ii) Many of the cell lines also contained variable amounts of non-tk gene viral DNA sequences located between map coordinates 0.11 to 0.57 and 0.82 to 1.00, suggesting that incorporation of the viral DNA sequences located between these map coordinates is a relatively random event. (iii) The viral DNA sequences located between map coordinates 0 to 0.11 and 0.57 to 0.82 were uniformly absent from all of the HSVtk+ cell lines tested, suggesting that there is a strong negative selective pressure against incorporation of these viral DNA sequences.

Original languageEnglish
Pages (from-to)272-285
Number of pages14
JournalJournal of Virology
Volume33
Issue number1
DOIs
StatePublished - 1980
Externally publishedYes

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