@article{76f964de22ba4be399525a8564bb5778,
title = "Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS",
abstract = "The 3′ region of a gene designated cipB, which shows strong homology with cipA that encodes the cellulosome SL subunit of Clostridium thermocellum ATCC 27405, was isolated from a gene library of C. thermocellum strain YS. The truncated S1 protein encoded by the cipB derivative bound tightly to cellulose. The cellulose-binding domain in this polypeptide consisted of a C-terminal proximal 167 residue sequence which showed complete identity with residues 337-503 of mature SL from C. thermocellum strain ATCC 27405. The cellulose-binding domain interacted with both crystalline and amorphous cellulose, but not with xylan.",
keywords = "Cellulase, Cellulose binding, Cellulosome, Clostridium thermocellum",
author = "Poole, \{Debbie M.\} and Ely Morag and Raphael Lamed and Bayer, \{Edward A.\} and Hazlewood, \{Geoffrey P.\} and Gilbert, \{Harry J.\}",
note = "Funding Information: We are gratefult o Drs. U.T. GerngrossA, .L. Demain and M.P.M. Romaniec for providing pre-publicatiodna ta.We also thank Sue Taylor for her excellent technicaasls istanceT.h is work was supportedb y the Agricultural anFodo d Re- searchC ouncil(Grant No. LE 13/138).E .M. was the recipient oaf scholarshipfr om the Foreign and CommonwealtOhf fice, and theC lore Foundation.",
year = "1992",
month = dec,
day = "1",
doi = "10.1111/j.1574-6968.1992.tb05563.x",
language = "אנגלית",
volume = "99",
pages = "181--186",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "2-3",
}