Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS

Debbie M. Poole, Ely Morag, Raphael Lamed, Edward A. Bayer, Geoffrey P. Hazlewood, Harry J. Gilbert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

The 3′ region of a gene designated cipB, which shows strong homology with cipA that encodes the cellulosome SL subunit of Clostridium thermocellum ATCC 27405, was isolated from a gene library of C. thermocellum strain YS. The truncated S1 protein encoded by the cipB derivative bound tightly to cellulose. The cellulose-binding domain in this polypeptide consisted of a C-terminal proximal 167 residue sequence which showed complete identity with residues 337-503 of mature SL from C. thermocellum strain ATCC 27405. The cellulose-binding domain interacted with both crystalline and amorphous cellulose, but not with xylan.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalFEMS Microbiology Letters
Volume99
Issue number2-3
DOIs
StatePublished - 1 Dec 1992

Funding

FundersFunder number
Agricultural anFodo d Re- searchC ouncil

    Keywords

    • Cellulase
    • Cellulose binding
    • Cellulosome
    • Clostridium thermocellum

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