Identification of sequences in the long terminal repeat of the lymphoproliferative disease virus required for efficient transcription

Ronit Sarid, Arnona Gazit, Steven R. Tronick, Abraham Yaniv

Research output: Contribution to journalArticlepeer-review

Abstract

We analyzed the long terminal repeat (LTR) of the lymphoproliferative disease virus of turkeys for sequences that influence its promoter activity by using the chloramphenicol acelyltransferase assay. A series of LTR deletion mutants and recombinants between LTR and simian virus 40 regulatory sequences were used for these studies. Through transfection experiments, we identified a negative regulatory element residing at the 5' end of the U3. The two imperfect direct repeats (DRs) located at nt - 170 to - 125 upstream of the RNA transcription site were identified as enhancer elements which could stimulate transcription of a heterologous promoter in an orientation independent manner. Specific interaction of nuclear factors with the DRs element was identified. The two DRs contain cArg motifs which are suggested to play a role in tissue specific expression of several cellular genes.

Original languageEnglish
Article number71213
Pages (from-to)789-794
Number of pages6
JournalVirology
Volume208
Issue number2
DOIs
StatePublished - 20 Apr 1995

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