Identification of sequences encoding the equine infectious anemia virus tat gene

Silvia Noiman, Arnona Gazit, Orli Tori, Levana Sherman, Toru Miki, Steven R. Tronick, Abraham Yaniv

Research output: Contribution to journalArticlepeer-review

Abstract

Equine infectious anemia virus (EIAV), a lentivirus, encodes a trans-activator (tat) which stimulates gene expression directed by the viral long terminal repeat (LTR). This function has been previously shown by us and others to be encoded by sequences within the middle region of the EIAV genome in which two short open reading frames, S1 and S2, reside. In the present study, by using in vitro mutagenesis, we show that disruption of S1, but not S2, completely abolished trans-activation. Addition of oligonucleotides complementary to S1 to cells transfected with a tat expression vector resulted in inhibition of trans-activation. EIAV cDNAs were isolated from a library of EIAV-infected cells constructed by using a eukaryotic expression vector. One cDNA clone which contained S1 sequences was able to trans-activate the EIAV LTR. Sequence analysis of this cDNA clone revealed that, in addition to S1, two other open reading frames were present. The cDNA still retained its activity when the latter two sequences were deleted.

Original languageEnglish
Pages (from-to)280-288
Number of pages9
JournalVirology
Volume176
Issue number1
DOIs
StatePublished - May 1990

Fingerprint

Dive into the research topics of 'Identification of sequences encoding the equine infectious anemia virus tat gene'. Together they form a unique fingerprint.

Cite this