Identification of peanut agglutinin-binding glycoproteins on immature human thymocytes

Antonio De Maio*, Halina Lis, Jonathan M. Gershoni, Nathan Sharon

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Previous studies in our laboratory have shown that peanut agglutinin (PNA), a lectin specific for the disaccharide Galβ3GalNAc, binds to immature (cortical) thymocytes of mouse and man and not to the mature (medullary) cells. Using lectin overlay of protein blots and lectin-affinity chromatography, we have found that the major PNA-binding glycoproteins on total as well as on immature (PNA+) human thymocytes correspond to two bands of Mr 170,000 and 180,000. Another glycoprotein, of Mr 110,000, also binds PNA but to a lesser extent. All three glycoproteins contain sialic acid as demonstrated by cell surface labeling with NaIO4NaB3H4, binding of wheat germ agglutinin, and reaction with alkaline phosphatase-hydrazide. After treatment with sialidase, binding of PNA to these glycoproteins is significantly enhanced.

Original languageEnglish
Pages (from-to)345-353
Number of pages9
JournalCellular Immunology
Issue number2
StatePublished - May 1986
Externally publishedYes


Dive into the research topics of 'Identification of peanut agglutinin-binding glycoproteins on immature human thymocytes'. Together they form a unique fingerprint.

Cite this