Identification of palindromic sequences recognized by restriction endonucleases, as based on the tabularized sequencing data for seven viral and plasmid DNAs

Camil Fuchs, Eric C. Rosenvold, Alik Honigman, Waclaw Szybalski*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

45 Scopus citations

Abstract

Computer search of DNA sequences for phages φX174, G4, M13 and fd, plasmids pBR322 and pA03, and virus SV40, was employed to prepare tables specifying the size classes and frequencies of DNA segments located between all possible tetra-, penta- and hexanucleotide palindromes. As described earlier (Fuchs et al., 1978), these tables permit identifying sequences recognized by most of the restriction endonucleases. The effect of sequencing errors on the accuracy of the present identification method is evaluated. Only four of the 224 listed sequences do not appear in any of the seven DNAs, leading to discussion (see Appendix) on the natural sequence distribution.

Original languageEnglish
Pages (from-to)357-370
Number of pages14
JournalGene
Volume10
Issue number4
DOIs
StatePublished - 1980
Externally publishedYes

Funding

FundersFunder number
National Cancer InstituteP30CA007175

    Keywords

    • Recombinant DNA
    • bacteriophages φ X174, G4, fd, M13
    • computer search
    • pA03
    • plasmids pBR322
    • tetra-, penta-, hexanucleotides
    • virus SV40

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