Identification of histidine-rich glycoprotein, a potential autoantigen, in human and rat brain preparations

Catrin Ball-Rosen, Hagit Primak-Rubinovicz, Amos D. Korczyn, Ningshan Wang, Adel Farchi, Sara Shavit, Yehuda Shoenfeld, Joab Chapman

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

β2-glycoprotein-I (β2-GPI, also known as apolipoprotein H) is a major autoantigen in the antiphospholipid syndrome (APS), a disease commonly affecting the central nervous system. We examined whether β2-GPI and similar proteins exist in rat and human brains. No expression was found on Northern blot analysis of human brain. Utilizing a standard procedure for the isolation of serum β2-GPI we purified a 100 kD human brain protein, which was found by peptide sequencing to have full homology with the serum protein, histidine-rich glycoprotein (HRGP). Expression of HRGP in rat and human brain was established by RT-PCR studies and a partial sequence of rat brain HRGP was obtained showing 68% homology with the human protein. IgG from most APS patients bound to HRGP, which shares distinct biochemical properties with β2-GPI, is present in the brain and may be an important autoantigen.

Original languageEnglish
Title of host publicationAutoimmunity, Part A Basic Principles and New Diagnostic Tools
PublisherBlackwell Publishing Inc.
Pages473-483
Number of pages11
ISBN (Print)1573316636, 9781573316637
DOIs
StatePublished - Aug 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1109
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Antiphospholipid antibodies
  • Brain
  • Histidine-rich glycoprotein
  • β-glycoprotein

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