TY - GEN
T1 - Identification of histidine-rich glycoprotein, a potential autoantigen, in human and rat brain preparations
AU - Ball-Rosen, Catrin
AU - Primak-Rubinovicz, Hagit
AU - Korczyn, Amos D.
AU - Wang, Ningshan
AU - Farchi, Adel
AU - Shavit, Sara
AU - Shoenfeld, Yehuda
AU - Chapman, Joab
PY - 2007/8
Y1 - 2007/8
N2 - β2-glycoprotein-I (β2-GPI, also known as apolipoprotein H) is a major autoantigen in the antiphospholipid syndrome (APS), a disease commonly affecting the central nervous system. We examined whether β2-GPI and similar proteins exist in rat and human brains. No expression was found on Northern blot analysis of human brain. Utilizing a standard procedure for the isolation of serum β2-GPI we purified a 100 kD human brain protein, which was found by peptide sequencing to have full homology with the serum protein, histidine-rich glycoprotein (HRGP). Expression of HRGP in rat and human brain was established by RT-PCR studies and a partial sequence of rat brain HRGP was obtained showing 68% homology with the human protein. IgG from most APS patients bound to HRGP, which shares distinct biochemical properties with β2-GPI, is present in the brain and may be an important autoantigen.
AB - β2-glycoprotein-I (β2-GPI, also known as apolipoprotein H) is a major autoantigen in the antiphospholipid syndrome (APS), a disease commonly affecting the central nervous system. We examined whether β2-GPI and similar proteins exist in rat and human brains. No expression was found on Northern blot analysis of human brain. Utilizing a standard procedure for the isolation of serum β2-GPI we purified a 100 kD human brain protein, which was found by peptide sequencing to have full homology with the serum protein, histidine-rich glycoprotein (HRGP). Expression of HRGP in rat and human brain was established by RT-PCR studies and a partial sequence of rat brain HRGP was obtained showing 68% homology with the human protein. IgG from most APS patients bound to HRGP, which shares distinct biochemical properties with β2-GPI, is present in the brain and may be an important autoantigen.
KW - Antiphospholipid antibodies
KW - Brain
KW - Histidine-rich glycoprotein
KW - β-glycoprotein
UR - http://www.scopus.com/inward/record.url?scp=34848837391&partnerID=8YFLogxK
U2 - 10.1196/annals.1398.053
DO - 10.1196/annals.1398.053
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C2 - 17785336
AN - SCOPUS:34848837391
SN - 1573316636
SN - 9781573316637
T3 - Annals of the New York Academy of Sciences
SP - 473
EP - 483
BT - Autoimmunity, Part A Basic Principles and New Diagnostic Tools
PB - Blackwell Publishing Inc.
ER -
