TY - JOUR
T1 - Identification of glycoproteins that are receptors for peanut agglutinin on immature (cortical) mouse thymocytes
AU - De Maio, Antonio
AU - Lis, Halina
AU - Gershoni, Jonathan M.
AU - Sharon, Nathan
N1 - Funding Information:
This study was supportedb y the United States-Israel Binational Science Foundation (grant no.2887/82)a nd the Hermann und Lilly Schilling Stiftung im Stifterverband.
PY - 1986/1/1
Y1 - 1986/1/1
N2 - Binding of peanut agglutinin is being widely used as a marker for immature mouse thymocytes and for the separation of these cells from the mature thymocytes. Two cell surface glycoproteins that bind peanut agglutinin were detected on unfractionated as well as immature thymocytes by lectin overlay and affinity chromatography: one of Mr between 170000 and 180000, and the other, a minor component, of Mr 110000, both of which are partially sialylated. No receptors for peanut agglutinin were detected on the mature cells, whereas desialylation experiments revealed the presence of a glycoprotein of Mr 110000. These findings were corroborated by electrophoretic analysis of cell surface glycoproteins of the isolated thymocyte subpopulations labeled in their carbohydrate moieties.
AB - Binding of peanut agglutinin is being widely used as a marker for immature mouse thymocytes and for the separation of these cells from the mature thymocytes. Two cell surface glycoproteins that bind peanut agglutinin were detected on unfractionated as well as immature thymocytes by lectin overlay and affinity chromatography: one of Mr between 170000 and 180000, and the other, a minor component, of Mr 110000, both of which are partially sialylated. No receptors for peanut agglutinin were detected on the mature cells, whereas desialylation experiments revealed the presence of a glycoprotein of Mr 110000. These findings were corroborated by electrophoretic analysis of cell surface glycoproteins of the isolated thymocyte subpopulations labeled in their carbohydrate moieties.
KW - Blot analysis Glycoprotein Peanut agglutinin Receptor Thymocyte subpopulation
UR - http://www.scopus.com/inward/record.url?scp=0022633401&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(86)80045-X
DO - 10.1016/0014-5793(86)80045-X
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AN - SCOPUS:0022633401
VL - 194
SP - 28
EP - 32
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -