Identification of feline- and canine-like rotaviruses isolated from humans by restriction fragment length polymorphism assay

A. Vonsover*, I. Shif, I. Silberstein, H. Rudich, Y. Aboudy, E. Mendelson, L. Shulman, T. Nakagomi, O. Nakagomi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Restriction fragment length polymorphism assay of reverse-transcribed and polymerase chain reaction-amplified rotavirus gene segment 9 was developed to differentiate human serotype 3 rotaviruses from animal serotype 3 rotaviruses. On the basis of similarities or differences in HinfI and DdeI restriction profiles, unusual group A serotype 3 human rotaviruses that belonged to subgroup I were shown to be of feline and canine origin. By this approach, the new human rotavirus isolates 5193, AU-387, AU-720, AU-785, and AU-1115 were shown to resemble certain feline-like human rotaviruses. Similar results were previously obtained by Nakagomi et al. (O. Nakagomi, A. Hoshima, Y. Aboudy, I. Shif, M. Mochizuki, T. Nakagomi, and T. Gotlieb-Stematsky, J. Clin. Microbiol. 28:1198-1203, 1990) by using RNA-RNA cross hybridization with established feline rotaviruses. The restriction fragment length polymorphism assay can provide fast and valuable information on the interspecies transmission of rotaviruses in nature.

Original languageEnglish
Pages (from-to)1783-1787
Number of pages5
JournalJournal of Clinical Microbiology
Volume31
Issue number7
DOIs
StatePublished - 1993
Externally publishedYes

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